• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of genetics >Identification and expression profiling of MSY genes of yak for bull fertility
【24h】

Identification and expression profiling of MSY genes of yak for bull fertility

机译：武力牦牛MSY基因的鉴定与表达分析

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Yak (Bos grunniens) is a unique bovine species and considered as lifeline of highlanders. The male subfertility in yak is a matter of concern that causes huge economic loses. The spermatogenesis and male reproduction machinery are critically governed by Y-linked genes which tend to acquire necessary information in the course of evolution. The Y-linked fertility genes are present in multiple copies with testis-limited expression. To understand this novel complexity, 12 male-specific region of Y chromosome (MSY) genes have been studied in the yak. Targeted genes are amplified in male and female genomic DNA and confirmed the male derived specificity. Moreover, testis and sperm-specific expressions of MSY genes are distinct among different tissues. The quantitative polymerase chain reaction results validate the expression pattern of these genes in various tissues with predominant expression intestis and sperm. The sequencing of resultant yak MSY genes gives significant result and shows similarity with cattle (Bos indicus), but few nucleotide mismatches define the proposition of infertile male in the F1 hybrid of cattle and yak. The identified MSY genes can be used to establish male-specific characteristics and to differentiate male and female yak genotypically. Further, these genes may act as valuable resources to understand the capacity of spermatogenesis, embryogenesis, cellular growth, azoospermia and malesubfertility in the yak.
机译：Yak（Bos Grunniens）是一种独特的牛种类，被认为是高地的生命线。牦牛的雄性化学者是一个令人担忧的问题，导致巨大的经济失败。精子发生和雄性繁殖机械受到y键入基因的严重管辖，倾向于在进化过程中获得必要的信息。 Y键的生育基因存在于多个拷贝中，具有睾丸有限的表达。为了了解这种新的复杂性，在牦牛中已经研究了12个阳性染色体（MSY）基因的阳性区域。靶向基因在雄性和雌性基因组DNA中扩增，并证实了雄性衍生的特异性。此外，MSY基因的睾丸和精异特异性表达在不同的组织中是不同的。定量聚合酶链反应结果验证具有主要表达肠和精子的各种组织中这些基因的表达模式。结果yak MSY基因的测序产生了显着的结果，并与牛（Bos indicus）表示相似之处，但很少有核苷酸错配在牛和牦牛的F1杂交中定义不育的雄性的命题。鉴定的MSY基因可用于建立男性特异性，并区分雄性和雌性牦牛基因型。此外，这些基因可以充当宝贵的资源，以了解牦牛中的精子发生，胚胎发生，细胞生长，厌氧植物和羟乙症和羟脲的能力。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号