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首页> 外文期刊>Journal of genetics >Molecular cloning, expression and mimicking antiviral activity analysis of retinoic acid-inducible gene-I in duck (Anas platyrhynchos)
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Molecular cloning, expression and mimicking antiviral activity analysis of retinoic acid-inducible gene-I in duck (Anas platyrhynchos)

机译：视黄酸 - 诱导基因-1中鸭（ANAs platyrhynchos）的分子克隆，表达和模拟抗病毒活性分析

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Intracellular double-stranded RNA (dsRNA) is a chief sign of replication for many viruses. Pattern recognition receptors(PRRs) of the innate immune system detected the dsRNA and initiate the antiviral responses. Retinoic acid-inducible gene I (RIG-I), a member of PRRs, plays an essential regulatory role in dsRNA-induced signalling. In this study, the full-length complementary DNA (cDNA) of duck RIG-I (duRIG-I) was cloned using the reverse transcription-polymerase chain reaction (RT-PCR) and rapid amplification of the cDNA ends (RACE). The cDNA of duRIG-I contained 97-bp 5'UTR, 141-bp 3'-UTR and 2802 bp complete open-reading frame (ORF) encoding 933 amino acids. Multiple sequence alignments showed that duRIG-I shared high similarity with RIG-I from other vertebrates. Quantitative real-time PCR (qRT-PCR) analysis revealed that duRIG-I mRNA was expressed in all tested tissues, with high levels in the liver, heart, spleen, kidney and thymus, while lower in the duodenum. duRIG-I could be induced by treatment with poly(I:C). Further, overexpression of duRIG-I significantly activated the transcription of poly(I:C)-induced IFN-b, IRF7, TRIF, Mx, STAT1 and STAT2 mRNA, and duRIG-I knockdown showed the opposite results. Overall, our results suggested that duRIG-I could be an important receptor for mimicking antiviral state in duck, which warrant further studies to show the possible mechanism.
机译：细胞内双链RNA（DSRNA）是许多病毒复制的主要标志。先天免疫系统的图案识别受体（PRRS）检测到DSRNA并引发抗病毒反应。 RETINICIC酸 - 诱导基因I（RIG-I）是PRR的成员，在DSRNA诱导的信号传导中起重要的调节作用。在该研究中，使用逆转录 - 聚合酶链反应（RT-PCR）克隆鸭钻型I（DURIG-I）的全长互补DNA（cDNA），并快速扩增cDNA末端（种族）。 DURIG-I的cDNA包含97-BP 5'UTR，141-BP 3'-UTR和2802bp完全开放阅读框（ORF）编码933氨基酸。多个序列对齐显示DURIG-I与来自其他脊椎动物的钻机I共享高相似性。定量实时PCR（QRT-PCR）分析显示，Durig-I mRNA在所有测试组织中表达，肝脏，心脏，脾，肾和胸腺中的高水平，而在十二指肠中较低。 DURIG-I可以通过聚（I：C）治疗诱导。此外，Durig-i的过表达显着激活了聚（I：c）的转录 - 诱导的IFN-B，IRF7，TRIF，MX，Stat1和Stat2 mRNA，并且Durig-I敲低显示相反的结果。总体而言，我们的结果表明，Durig-i可以成为模仿鸭子抗病毒状态的重要受体，这需要进一步研究以显示可能的机制。

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