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首页> 外文期刊>Journal of genetics >Single-nucleotide polymorphisms in CLEC7A, CD209 and TLR4 gene and their association with susceptibility to paratuberculosis in Indian cattle
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Single-nucleotide polymorphisms in CLEC7A, CD209 and TLR4 gene and their association with susceptibility to paratuberculosis in Indian cattle

机译：CLEC7A，CD209和TLR4基因中的单核苷酸多态性及其与印度牛的抗麻痹性易感性的关联

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The aim of this study was to identify the single-nucleotide polymorphisms (SNPs) in bovine candidate genes CLEC7A, CD209 and TLR4, and explore the association between these SNPs with the occurrence of bovine paratuberculosis (PTB) disease. For this purpose, 549 animals were screened by a panel of four diagnostic tests, namely Johnin PPD test, ELISA test, faecal microscopy and IS900 blood PCR against Mycobacterium avium ssp. paratuberculosis (MAP) to develop casea??control populations. SNPs were genotyped by polymerase chain reaction-restriction fragment length polymorphism method. Genotypica??phenotypic associations were assessed by the PROCLOGISTIC procedure of SAS 9.3. Of the seven SNPs; rs110353594 in CLEC7A gene and rs8193046 in TLR4 gene were found to be associated with PTB. For SNP rs110353594, odds of CC and CT genotypes vs TT genotype was 1.543 (0.420a??5.667; 95% CI) and 0.284 (0.104a??0.774; 95% CI), respectively which means that CT genotype was more resistant than TT and CC genotypes against bovine PTB. For SNP rs8193046, odds of AA and AG genotypes versus GG genotype was 0.947 (0.296a??3.034; 95% CI) and 3.947 (1.555a??10.022; 95% CI), respectively, i.e. probability for getting an infection in animals with AG genotype was 3.94 times more as compared to GG genotype. Hence, a selection programme favouring CT genotype for rs110353594 and against AG genotype for rs8193046 may be beneficial for conferring resistance against bovine PTB.
机译：该研究的目的是鉴定牛候选基因Clec7a，CD209和TLR4中的单核苷酸多态性（SNP），并探讨这些SNP之间的关联，随着牛解霉（PTB）疾病的发生。为此目的，通过四个诊断测试面板筛选549只动物，即Johnin PPD测试，ELISA测试，粪便显微镜和IS900血液PCR针对分枝杆菌SSP。 Paratuberculosis（地图）开发Casea的控制人口。通过聚合酶链反应限制片段长度多态性方法进行SNP进行基因分型。通过SAS 9.3的促进程序评估基因孔的表型协会。七个snps;发现CLEC7A基因的RS110353594和TLR4基因的RS8193046与PTB相关。对于SNP RS110353594，CC和CT基因型对TT基因型的几率分别为1.543（0.420A？5.667; 95％CI）和0.284（0.104A〜0.774; 95％CI），这意味着CT基因型比TT和CC基因型对牛PTB。对于SNP RS8193046，AA和AG基因型的几率与GG基因型为0.947（0.296A？3.034; 95％CI）和3.947（1.555A？10.022; 95％CI），即在动物中感染概率与GG基因型相比，Ag基因型为3.94倍。因此，有利于RS110353594的CT基因型的选择程序和RS8193046对抗Ag基因型可能是有益于赋予牛PTB的抵抗力。

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