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首页> 外文期刊>Journal of genetics >Genomewide analysis of the CIII peroxidase family in sweet orange (Citrus sinensis) and expression profiles induced by Xanthomonas citri subsp. citri and hormones
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Genomewide analysis of the CIII peroxidase family in sweet orange (Citrus sinensis) and expression profiles induced by Xanthomonas citri subsp. citri and hormones

机译：甜橙（柑橘Sinensis）中C 1II过氧化物酶系列的基因组分析及Xanthomonas Citri子阶段诱导的表达谱。 Citri和荷尔蒙

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Class III peroxidase (CIII prx) is a plant-specific multigene family that regulates the physiological and stress responses. This research aimed to exhaustively annotate and analyse the CIII prx family in sweet orange and to explore the regulated expression profiles by Xanthomonas citri subsp. citri (Xcc) and plant hormones. We further assessed the relationship between CIII prxs and citrus bacterial canker. The phylogeny, gene structure, conserved motifs, gene duplications and microsynteny of the CIII prx family were analysed. Expression profiles of specific CsPrxs induced by Xanthomonas citri subsp. citri and plant hormones were detected by quantitative reverse transcription-polymerase chain reaction. Subcellular localization was analysed through transient expression assessments. A total of 72 CIII prx members were identified from the genomes of sweet orange. In all chromosomes of sweet orange, the CsPrxs could be detected except in chromosome 8. In addition, three segmental duplications, four tandem duplications and 11 whole-genome duplications occurred among the CsPrxs, contributing to the family size expansion. From the Ka/Ks ratios, 15 of 18 duplicated CsPrxs pairs have experienced purifying selection process. A total of 15 conserved motifs were detected in CsPrxs, four of which were detected in all complete CsPrxs. A total of 12 expressed genes were identified from the EST database. The expression trends of 12 CsPrxs were differently expressed at different stages of infection by Xcc, five of which were potential candidate genes involved in Xcc resistance. These genes could be induced by salicylic acidand methyl jasmonate, and were extracellular proteins. These results further support our understanding of CIII prxs in citrus, particularly incitrus bacterial canker studies.
机译：III类过氧化物酶（CIII PRX）是一种植物特异性的多烯家族，用于调节生理和应激反应。这项研究旨在详尽的注释和分析甜橙中的CIII PRX家族，并探讨Xanthomonas Citri Subsp的调节表达谱。 Citri（XCC）和植物激素。我们进一步评估了CIII PRX和柑橘细菌腐肉之间的关系。分析了细胞发育，基因结构，保守的基序，基因重复和CIII PRX系列的微生物。 Xanthomonas Citri子公司诱导特异性CSPRX的表达谱。通过定量逆转录聚合酶链反应检测Citri和植物激素。通过瞬态表达评估分析亚细胞定位。共有72种CIII PRX成员被鉴定出甜橙的基因组。在甜橙的所有染色体中，除了染色体8中，可以检测到CSPRX。此外，CSPRX中的三个节段重复，四个串联重复和11个全基因组重复，有助于家庭规模扩张。从KA / KS比率，18个重复的CSPRX对中的15个具有经验丰富的净化选择过程。在CSPRX中检测到总共15个保守的基序，其中四个在所有完整的CSPRX中检测到其中。从EST数据库中鉴定了总共12种表达基因。 12个CSPRX的表达趋势在XCC的不同感染阶段不同地表达，其中5个是涉及XCC抗性的潜在候选基因。这些基因可以通过水杨酸和茉莉酸甲酯诱导，并且是细胞外蛋白质。这些结果进一步支持我们对柑橘中CIII PRX的理解，特别是侵袭性细菌溃疡研究。

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