• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of King Saud University >Exploring the molecular mechanism of Panax notoginseng saponins on coronary atherosclerosis let-7b using miRNA
【24h】

Exploring the molecular mechanism of Panax notoginseng saponins on coronary atherosclerosis let-7b using miRNA

机译：使用miRNA探索冠状动脉粥样硬化Let-7b冠状动脉粥样硬化Sapon的分子机制

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

ObjectiveThe miRNA was used to explore the molecular mechanism and effects of Panax notoginseng saponins (PNS) on coronary atherosclerosis let-7b and understand the variations in let-7b molecular mechanism after the application of PNS, which verified the therapeutic effects of PNS on coronary atherosclerosis and provided guidance for the clinical treatment and diagnosis of coronary atherosclerosis. Methods: Patients who were clinically diagnosed as coronary atherosclerosis in the hospital were taken as research objects and divided into two groups, i.e. the experiment (treatment) group, and the control group. The peripheral venous blood sample of each patient was taken in the morning on an empty stomach to extract the total RNA. In addition, patients in the two groups received symptomatic treatments, such as anti-ischemic therapy, anti-platelet therapy, and hypo-lipidemic therapy. Simultaneously, patients in the treatment group took Xuesaitong Capsule, while patients in the control group took the simulant Xuesaitong Capsule. After four weeks, blood samples were drawn again to extract the total RNA. Then, the target gene and related pathways of let-7b were determined by using the micro RNA target gene prediction software miRBase. Finally, the real-time fluorescence quantitative polymerase chain reaction (QRT-PCR) was used to investigate the effect of PNS on the molecular mechanism of coronary atherosclerosis let-7b. Results: Both let-7b and ADRB2 were up-regulated after treatment in the treatment group, while the let-7b and ADRB2 were down-regulated after treatment in the control group. In addition, the levels of molecules in the treatment group were down-regulated, including GS, AC, cAMP, PKA, and PLIN3, which indicated that PNS might regulate the downstream signaling pathway through let-7b/ADRB2 to achieve the therapeutic effects. Conclusion: The study found that the application of PNS could up-regulate let-7b and ADRB2 in patients with unstable angina pectoris, indicating that PNS functioned through let-7b and ADRB2 channels, such as protecting ischemia reperfusion of myocardial cells, mobilizing the bone marrow stem cells for angiogenesis, improving the survival rate after transplantation of bone marrow mesenchymal stem cells, reducing the apoptosis of myocardial cells, and promoting the repair of myocardial-related protein expressions.
机译：客观的MiRNA用于探讨Panax Notinseng Saponins（PNS）对冠状动脉粥样硬化Let-7B的分子机制和作用，并了解PNS后Let-7B分子机制的变化，这验证了PNS对冠状动脉粥样硬化对冠状动脉粥样硬化的治疗效果并为冠状动脉粥样硬化的临床治疗和诊断提供了指导。方法：临床诊断为冠状动脉粥样硬化的患者作为研究对象，分为两组，即实验（治疗）组和对照组。每人患者的外周静脉血液样品在空腹上拍摄，以提取总RNA。此外，两组患者在两组接受症状治疗，如抗缺血治疗，抗血小板治疗和缺血性疗法。同时，治疗组患者服用XuedaAitong胶囊，而对照组的患者采用了模拟XuedaAitong胶囊。四周后，再次抽取血样以提取总RNA。然后，通过使用微RNA靶基因预测软件MiRBase来确定Let-7B的靶基因和相关途径。最后，使用实时荧光定量聚合酶链反应（QRT-PCR）来研究PNS对冠状动脉粥样硬化Let-7B的分子机制的影响。结果：在治疗组中治疗后，Let-7B和ADRB2都上调，而在对照组的处理后，Let-7B和ADRB2下调。此外，治疗组中的分子水平被下调，包括GS，AC，CAMP，PKA和PLIN3，表明PNS可以通过Let-7B / ADRB2调节下游信号通路以实现治疗效果。结论：该研究发现，PNS的应用可以在不稳定的心绞痛患者患者上调节Let-7B和ADRB2，表明通过Let-7B和ADRB2通道发挥的PN，例如保护心肌细胞的缺血再灌注，动员骨骼骨髓干细胞用于血管生成，改善骨髓间充质干细胞移植后的存活率，降低心肌细胞的凋亡，促进心肌相关蛋白表达的修复。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号