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首页> 外文期刊>Journal of King Saud University >Molecular identification of herbal species belonging to genus Piper within family Piperaceae from northern Peninsular Malaysia
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Molecular identification of herbal species belonging to genus Piper within family Piperaceae from northern Peninsular Malaysia

机译：属于Genus的草药物种的分子鉴定来自北半岛马来西亚的哌科西

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Species identification technology (i.e. DNA barcoding) has been recognized as an important and reliable tools, although the effectiveness of using a single universal barcode marker for plants is still questionable. The present study reports a molecular identification method to discriminate 11 plant species within genusPiperthat extensively utilized in traditional healing practices in Malaysia. The plants were sampled from six locations that represents three states in northern Peninsular of Malaysia, namely Perlis, Kedah and Penang. Species discrimination was conducted using BLASTn analysis and phylogenetic inference based on Maximum Likelihood and Neighbour-Joining method for two target genes,rbcLandrpoC1. The reliability of phylogenetic tree regenerations was assessed using Disparity Index analysis. Estimation of evolutionary divergence between all samples was employed based on Maximum Composite Likelihood with Kimura 2-parameter model. The present study revealed that the DNA identification method has successfully discriminated all samples to species level and that therbcLis the reliable marker for identification. The outcome of the present study gives a significant information on the DNA barcoding of plants within genusPiper.
机译：物种识别技术（即DNA条形码）已被认为是一个重要且可靠的工具，尽管使用用于植物的单个通用条形码标记的有效性仍然是可疑的。本研究报告了分子鉴定方法，以区分11种植物物种在马来西亚的传统治疗实践中广泛使用。从六个地点取样植物，该地点代表马来西亚北部半岛的三种状态，即Perlis，Kedah和Penang。使用Blastn分析和系统发育基于用于两个靶基因的最大可能性和邻近接合方法，进行物种辨别，RBClandRPoc1。利用差异指数分析评估了系统发育树再生的可靠性。基于与Kimura 2参数模型的最大复合可能性，使用所有样品之间的进化分歧的估计。本研究表明，DNA鉴定方法已成功地将所有样品鉴定为物种水平，并且该方法将其鉴定可靠标记物。本研究的结果提供了关于Genuspiper内植物的DNA条形码的重要信息。

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