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首页> 外文期刊>Journal of King Saud University >Optimization of regeneration and Agrobacterium tumefaciens-mediated transient transformation systems for Australian native extremophile, Tripogon loliiformis
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Optimization of regeneration and Agrobacterium tumefaciens-mediated transient transformation systems for Australian native extremophile, Tripogon loliiformis

机译：优化再生和土壤杆菌肿瘤和斜视：斜体>介导的瞬态转换系统，用于澳大利亚原生Engentiophile， Tripogon loliiformis

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Tripogon loliiformis,an Australian native resurrection grass having an abundant gene pool for combating desiccation, can be the putative model system for functional characterization of stress tolerance genes due to its diploid genome and being a monocotyledonous plant and member of the grass family (Poaceae), like many important cereal crops. For developing callus mediated regeneration from mature grains ofTripogon, Murashige and Skoog medium containing growth regulator, 2,4-dichlorophenoxyacetic acid (2,4-D) at 1.0mgL?1was the optimum concentration for induction and proliferation of healthy cream calli. Successful regeneration of shoots from callus clumps in MS medium supplemented with 2.0mgL?16-benzylaminopurine (BAP) and 0.5mgL?1α-naphthalene acetic acid (NAA) was obtained from 2 consecutive rounds subculturing of the calli at 3?weeks interval. In addition, rooting needed another 2 rounds within the same media with 2.0mgL?1BAP but with 0.25mgL?1NAA. The transient expression ofUidAgene at 3?days afterTripogoncallus transformation, performed withAgrobacterium tumefaciensstrains AGL1 and LBA4404 following rice andBrachypodium distachyontransformation protocols, indicates successfulAgrobacteriuminfection and gene delivery in calli. A stable transformation system forTripogon loliiformiscan be developed near future following the protocols in this study.
机译：Tudogon loliiformis，一种用于打击干燥的丰富基因库的澳大利亚本地复活草，可以是由于其二倍体基因组，并且是单子叶植物和草家庭成员（Poaceae）的胁迫耐受基因功能表征的推定模型系统。像许多重要的谷物作物一样。用于在含有生长调节剂的成熟晶粒，Murashige和Skoog培养基中的愈伤组织介导的再生，含有生长调节剂，2,4-二氯苯氧基乙酸（2,4-D）在1.0mg1中的诱导和增殖的最佳浓度，健康乳腺癌。从补充有2.0mglα的MS培养基中的愈伤组织群中的芽的成功再生于3？周的愈伤组织的连续转移到愈伤组织中，得到了愈伤组织培养基（Bap）和0.5mglα1α1α-萘乙酸（NAA）。此外，在同一媒体内生根需要2.0mglΔ1bap，但含0.25mgl？1naa。乌丁蛋白的瞬时表达3？天后的后肺癌癌，在水稻和Brachylyntransfransformation方案中进行了多久进行了umefaciensstrains Agl1和LBA4404进行的，表明了Calli的成功突出僵菌和基因递送。在本研究的协议之后，将在未来开发稳定的转换系统Fortipogon Loliiformiscan。

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