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首页> 外文期刊>Journal of King Saud University >Enhanced production, purification and biochemical characterization of therapeutic potential fibrinolytic enzyme from a new Bacillus flexus from marine environment
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Enhanced production, purification and biochemical characterization of therapeutic potential fibrinolytic enzyme from a new Bacillus flexus from marine environment

机译：从海洋环境中提高来自新芽孢杆菌的治疗潜在纤维蛋白溶解酶的生产，纯化和生化特征

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ObjectivesThe main aim of this study is to isolate and characterize fibrinolytic enzyme from Bacillus flexus.MethodsFish meal ofSardinella longicepsand anchovy was optimized using a two-level full factorial design (25) and response surface methodology. The significant physical factors and nutrient sources (peptone, maltose, and magnesium chloride) were identified by statistical approach. The properties of a purified enzyme including their effect at different temperature, pH and the effect of metal ions were evaluated.ResultsEnzyme yield was improved 3.5 fold than unoptimized medium. Central composite design optimized culture medium enhanced enzyme yield (4711?±?29.3 U/g of substrate). The fibrinolytic enzyme was highly active at alkaline pH (8.0), 50?°C and the molecular weight was 32?kDa.ConclusionsFrom these findings, it concludes that this fibrinolytic enzyme could be a novel potent thrombolytic agent.
机译：本研究的主要目的是分离和表征来自芽孢杆菌的纤维蛋白溶解酶。使用两级全部造成设计（25）和响应表面方法，优化了肺炎的纤维蛋白酶。通过统计方法鉴定了显着的物理因素和营养源（蛋白胨，麦芽糖和氯化镁）。评估纯化酶的性质，包括它们在不同温度，pH和金属离子的作用下的效果。结果酶产量比未优化的介质提高3.5倍。中央复合设计优化培养基增强酶产率（4711？±29.3 u / g基板）。纤维蛋白溶解酶在碱性pH（8.0）中具有高活性，50Ω·℃，分子量为32μm≤kDa.Conclusions，它得出结论，该纤维蛋白溶解酶可能是一种新型有效血栓溶解剂。

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