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首页> 外文期刊>Journal of King Saud University >Heterologous WRKY and NAC transcription factors triggered resistance in Nicotiana benthamiana
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Heterologous WRKY and NAC transcription factors triggered resistance in Nicotiana benthamiana

机译：异源腕骨和NAC转录因子引发抗性尼古利亚纳·宾夕法尼亚州人和斜体>斜体>

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BackgroundSA (Salicylic acid) and JA (Jasmonic acid)/ET (Ethylene) are the defense and growth hormone regulators involved in alleviating the biotic and abiotic stresses.WRKYandNACgenes are contributors to plant resistance due to their active role in SA and JA/ET defense mechanism.MethodsWRKY and NAC transcription factors (ptHR293andptHR759, respectively) were serially selected (by performing cDNA library functional screening, homology analysis, antioxidant enzymes, ROS burst, callose deposition and qRT-PCR analysis) fromPinellia ternataand transformed intoNicotiana benthamiana. Hybrids were generated to analyze the stability of disease resistance. UPLC-QTOF-MS was performed to study the bioactive compounds.ResultsStudy demonstrated thatptHR293andptHR759had potential to trigger ROS burst and callose deposition inN. benthamianatogether with the activation of PR-genes and antioxidant enzymes. In transformedN. benthamiana, relative expression ofPR-1a(Pathogenesis related-1a) andPDF1.2(Plant defensin 1.2) was upregulated upto 21 folds and 4 folds forptHR293transformedN. benthamiana. WhilePR-1aandPDF1.2exhibited 8 folds and 26 folds upregulation forptHR759transformedN. benthamiana.ptHR293?+?ptHR759-F1hybrids also exhibited a significant level of PR-gene expression. Significantly high resistance againstBotrytis cinereawithout influencing the standard seed germination, root and shoot length of transformedN. benthamianawas also observed. A significant induction of bioactive compounds was also observed inptHR293transformedN. benthamiana.ConclusionConclusively, heterologous transcription factors,ptHR293andptHR759, perform their specific role in the activation of SA and JA/ET mediated defense mechanism.
机译：Backgroundsa（水杨酸）和Ja（茉莉酸）/等（乙烯）是涉及缓解生物和非生物胁迫的防御和生长激素调节因素。由于其在SA和JA / ET防御中，由于其活跃作用，因此是植物抗性的贡献者机制。均等选择了赤列和NAC转录因子（分别通过进行cDNA文库功能筛查，同源性分析，抗氧化酶，ROS爆发，胼internand和转化的Intonicotiana Benthamiana。产生杂交种以分析抗病抗性的稳定性。进行UPLC-QTOF-MS进行生物活性化合物。结果已经证明了Thr293andPthr759的潜力触发ROS爆发和胼intern。 Benthamianatogether伴有Pr-基因和抗氧化酶的激活。在转变。 Benthamiana，HER-1A（发病机制相关 - 1A）和PDF1.2（植物防御素1.2）的相对表达被上调高达21倍，4折293折形状。 Benthamiana。 wherpr-1aandpdf1.2申请8折和26倍上调投影措施。 Benthamiana.pthr293？+？Pthr759-F1hybrid也表现出显着的Pr-基因表达水平。显着高阻的抗rytytytytytis的变化影响转化的标准种子萌发，根和枝条长度。果树群岛也观察到。还观察到了生物活性化合物的显着诱导Inpthr293transformedn。 Benthamiana.ConclusionConclusive，异源转录因子，Pthr293andPthr759，在SA和JA / ET介导的防御机制的激活中进行其特定作用。

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