• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Integrative Bioinformatics >Nutrilyzer: A Tool for Deciphering Atomic Stoichiometry of Differentially Expressed Paralogous Proteins
【24h】

Nutrilyzer: A Tool for Deciphering Atomic Stoichiometry of Differentially Expressed Paralogous Proteins

机译：Nutrilyzer：用于解密差异表达旁透盲蛋白的原子化学计量的工具

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Summary Organisms try to maintain homeostasis by balanced uptake of nutrients from their environment. From an atomic perspective this means that, for example, carbon:nitrogen:sulfur ratios are kept within given limits. Upon limitation of, for example, sulfur, its acquisition is triggered. For yeast it was shown that transporters and enzymes involved in sulfur up- take are encoded as paralogous genes that express different isoforms. Sulfur deprivation leads to up-regulation of isoforms that are poor in sulfur-containing amino acids, that is, methinone and cysteine. Accordingly, sulfur-rich isoforms are down-regulated. We developed a web-based software, doped Nutrilyzer, that extracts paralogous protein coding sequences from an annotated genome sequence and evaluates their atomic compo- sition. When fed with gene-expression data for nutrient limited and normal conditions, Nutrilyzer provides a list of genes that are significantly differently expressed and simul- taneously contain significantly different amounts of the limited nutrient in their atomic composition. Its intended use is in the field of ecological stoichiometry. Nutrilyzer is available at http://nutrilyzer.hs-mittweida.de. Here we describe the work flow and results with an example from a whole-genome Ara- bidopsis thaliana gene-expression analysis upon oxygen deprivation. 43 paralogs distributed over 37 homology clusters were found to be significantly differently expressed while containing significantly different amounts of oxygen.
机译：总结生物试图通过平衡对环境的营养素的影响来维持稳态。从原子视角来，这意味着例如碳：氮：硫比在给定的限度内保持硫比率。在例如硫的限制后，触发其采集。对于酵母，表明参与硫的转运蛋白和酶被编码为表达不同同种型的副骨基因。硫剥夺导致含硫氨基酸中差的同种型的上调，即甲基酮和半胱氨酸。因此，富含硫的同种型是下调的。我们开发了一种基于Web的软件掺杂Nutrilyzer，该软件从注释的基因组序列中提取扑二甲蛋白质编码序列，并评估其原子伴侣。当喂入营养有限和正常条件的基因表达数据时，Nutrilyzer提供了一种基因列表，其显着表达，并在其原子组合物中含有显着不同量的有限营养素。其预期用途是生态化学计量领域。 Nutrilyzer可在http://nutrilyzer.hs-mittweida.de提供。在这里，我们描述了工作流程和结果，其中来自缺氧缺乏的全基因组ARA- Bidopsis的实例。 43分布在37种同源簇上的副葡萄糖被发现显着表达，同时含有显着不同的氧气。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号