• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Oral Microbiology >The metatranscriptomes of root caries and sound root surface biofilms
【24h】

The metatranscriptomes of root caries and sound root surface biofilms

机译：根龋齿和声根表面生物膜的metaTranscriptomes

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

There is limited knowledge of bacterial metabolism in root caries lesions. The aim of this study was to describe the bacterial metatranscriptomes associated with root caries and sound root surfaces using an RNA-seq analysis approach.The biofilms from exposed root surfaces were sampled from caries-free volunteers (n=10), and from the infected dentine of volunteers with root caries (n=30). Total bacterial RNA was extracted; cDNA libraries were prepared and sequenced on the Illumina Hi-Seq2500. The function and composition of the metabolically active microbiota were investigated using: a) MG-RAST, and b) denovo assembly of the read data and mapping to contigs. Differential gene expression analysis was done using the R package DESeq2 (padj 10?3).Transcripts with the highest expression levels were those coding for membrane transport systems, ribosomal proteins, enolase and glycolytic pathways in both groups. Differential analysis indicated that genes coding for the OmpA domain protein and metalloprote...
机译：根龋病患者的细菌代谢知识有限。本研究的目的是描述使用RNA-SEQ分析方法与根龋和声根表面相关的细菌跖展组。来自暴露根表面的生物膜从无龋志愿者（n = 10），来自受感染的生物膜志愿者的牙齿与根龋（n = 30）。提取总细菌RNA;制备cDNA文库并在Illumina Hi-SEQ2500上测序。使用：a）mg-rast和b）Denovo组装读取数据的函数和b）调查代谢活性微生物群的功能和组成，并将其映射到Contigs。使用r封装deseq2（padj <10-3）进行差异基因表达分析。具有最高表达水平的可用于两组中的膜输送系统，核糖体蛋白，烯醇酶和糖酵解途径的表达水平。差异分析表明，编码OMPA结构域蛋白和金属蛋白的基因......

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号