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首页> 外文期刊>Journal of Oral Microbiology >Modeling the development of proteolytic phenotypes in multi-species oral biofilms
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Modeling the development of proteolytic phenotypes in multi-species oral biofilms

机译：模拟多物种口腔生物膜中蛋白水解表型的发展

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In chronic periodontitis, subgingival biofilms induce an inflammatory response leading to periodontal tissue destruction which may cause tooth loss. The response includes exudation of fluid (GCF) from the gingival pocket, giving rise to a protein-rich micro-environment in the biofilm. We hypothesize that proteolytic activity in biofilms is a virulence factor contributing to sustained inflammation.To study the influence of GCF on proteolytic activity in a periodontitis-associated biofilm, a multi-species consortium (Porphyromonas gingivalis, Fusobacterium nucleatum, Parvimonas micra and Streptococcus constellatus) was grown in 10% equine serum (to model GCF) or BHI (control) for 2-5 days. Cell-associated and secreted proteolytic activity was investigated with zymography, fluorometry or fluorescent substrates in combination with fluorescence in situ hybridization (FISH).After 2 days, streptococci dominated the consortium in BHI whereas in serum diversity was maintained over 5 days. The serum consort...
机译：在慢性牙周炎中，龈下生物膜诱导炎症反应，导致可能导致牙齿损失的牙周组织破坏。响应包括从牙龈口袋的液体（GCF）渗出，从而产生生物膜中富含蛋白质的微环境。我们假设生物膜中的蛋白水解活性是有助于持续炎症的毒力因子。研究GCF在牙周炎相关生物膜中的蛋白水解活性的影响，多种联盟（卟啉核糖菌，核心核，Parvimonas micra和Streptococonas micra和streptococcus microblatus）在10％马血清（模拟GCF）或BHI（对照）中生长2-5天。将细胞相关和分泌的蛋白水解活性与致氮，荧光素或荧光基底一起研究，与荧光原位杂交（鱼）。在2天中，Streptococci占据了BHI的联盟，而在5天内保持血清多样性。血清联盟......

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