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首页> 外文期刊>Journal of Oral Microbiology >Biofilm behavior of Tannerella forsythia strains and S-layer glycosylation mutants
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Biofilm behavior of Tannerella forsythia strains and S-layer glycosylation mutants

机译：Tannerella连翘菌株和S层糖基化突变体的生物膜行为

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The periodontopathogen Tannerella forsythia has a characteristic cell surface (S-) layer modified with a unique O-glycan. This structure was analyzed for its role in biofilm formation employing an in vitro multispecies biofilm model, into which different T. forsythia strains and mutants with a modified cell surface composition were incorporated together with nine other oral species. The influence of the glycosylated T. forsythia S-layer on the bacterial composition of the biofilms was analyzed quantitatively using quantitative real-time PCR as well as qualitatively by fluorescence in situ hybridization and confocal laser scanning microscopy. It was evident that while changes of the T. forsythia cell surface did not affect the quantitative composition of the multispecies consortium, with the exception of Campylobacter rectus cell numbers, the localization of T. forsythia within the biofilm and its aggregation with Porphyromonas gingivalis were changed. Thus, the glycosylated T. forsythia S-layer mi...
机译：牙周病肠杆菌植物中的连翘症具有用独特的O-聚糖改性的特征细胞表面（S-）层。分析该结构的作用在使用体外多层的生物膜形成中的作用，其中与修饰细胞表面组合物的​​不同T.连续的菌株和突变体与九种其他口腔物种一起掺入其中。使用定量实时PCR分析糖基化的T.连翘的糖糖苷肽的影响在生物膜的细菌组合物上，以及原位杂交和共聚焦激光扫描显微镜的荧光分析。显然是，虽然T.连翘细胞表面的变化不影响多数联盟的定量组成，但弯角细胞数除外，生物膜内T.连翘内的定位及其与卟啉核苷酸的聚集进行了变化。因此，糖基化的T. forsythia s-layer mi ...

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