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Osteopontin adsorption to Gram-positive cells reduces adhesion forces and attachment to surfaces under flow

机译:对革兰氏阳性细胞的骨桥蛋白吸附可减少粘附力和在流动下的表面附着

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The bovine milk protein osteopontin (OPN) may be an efficient means to prevent bacterial adhesion to dental tissues and control biofilm formation. This study sought to determine to what extent OPN impacts adhesion forces and surface attachment of different bacterial strains involved in dental caries or medical device–related infections. It further investigated if OPN’s effect on adhesion is caused by blocking the accessibility of glycoconjugates on bacterial surfaces. Bacterial adhesion was determined in a shear-controlled flow cell system in the presence of different concentrations of OPN, and interaction forces of single bacteria were quantified using single-cell force spectroscopy before and after OPN exposure. Moreover, the study investigated OPN’s effect on the accessibility of cell surface glycoconjugates through fluorescence lectin-binding analysis. OPN strongly affected bacterial adhesion in a dose-dependent manner for all investigated species (Actinomyces naeslundii, Actinomyces viscosus,...
机译:牛奶蛋白骨骨桥蛋白(OPN)可以是预防细菌粘附到牙科组织和对照生物膜形成的有效手段。该研究试图确定OPN在多大程度上影响龋齿或医疗器械相关感染中不同细菌菌株的附着力和表面附着。它进一步研究了如果OPN对粘附的影响是通过阻断细菌表面上的糖缀合物的可替代性引起的。在存在不同浓度的OPN的存在下,在剪切控制的流动细胞系统中测定细菌粘附,并且在OPN暴露之前和之后使用单细胞力光谱定量单细胞的相互作用力。此外,该研究研究了OPN通过荧光凝集素结合分析对细胞表面糖缀合物的可及性的影响。 OPN强烈影响细菌粘附,以适应所有研究的物种(Actinomyces Naeslundii,放蛋白染色的......

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