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首页> 外文期刊>Journal of Sports Science and Medicine >Alteration of Autophagy Gene Expression by Different Intensity of Exercise in Gastrocnemius and Soleus Muscles of Wistar Rats
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Alteration of Autophagy Gene Expression by Different Intensity of Exercise in Gastrocnemius and Soleus Muscles of Wistar Rats

机译：Wistar大鼠胃肠炎和肌肉肌肌肌肉不同强度的自噬基因表达的改变

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Exercise-induced skeletal muscle adaptation requires degradation of cellular components carried out by autophagy. However, the alteration of autophagy by different intensity of exercise in skeletal muscle is still unknown. In the present study, we investigate whether low, moderate, and high-intensity exercises have different impacts on autophagy gene expression in gastrocnemius and soleus muscles of wistar rats. This work is limited because only rats are used, and does not cover human tissues. Twenty male wistar rats were assigned to four groups: sedentary control, low-intensity (LI, 10 m/minute), moderate-intensity (MI, 20 m/minute), and high-intensity (HI, 30 m/minute) exercises. Training was conducted 30 minutes/day with a 5 times/week interval for 8 weeks. RNA and protein were extracted from gastrocnemius and soleus muscles then stored in -80°C. Specific primers and antibodies for autophagy genes and protein levels were utilized for semi-quantitative PCR and Western Blot. Exercises decrease expression of autophagy gene LC3 (LI and MI 0.7 fold, HI 0.8 fold, p & 0.05) in gastrocnemius muscles and soleus muscle (LI, MI, and HI 0.8 fold, p & 0.05) compared to control. On the other side, we observed p62 gene expression decreased in gastrocnemius (0.8 fold, p & 0.05) and soleus (0.9 fold, p & 0.05) muscles with MI, but increased in soleus (1.1 fold, p & 0.05) muscles with HI. This result is consistent with the change of protein level, suggesting that autophagy might be modulated by different type of exercise. This study suggests that intensity of exercise and different type of muscle fibers effect autophagy gene expression in skeletal muscle of wistar rats. MI exercise increases autophagy gene expression in gastrocnemius and soleus muscles, but HI exercise decreases autophagy gene expression in soleus muscles of wistar rats. Soleus muscles are more responsive to exercise compared to gastrocnemius muscles.
机译：运动诱导的骨骼肌适应需要通过自噬进行细胞组分的降解。然而，骨骼肌中不同运动强度的自噬的改变仍然未知。在本研究中，我们研究了低，中等和高强度锻炼是否对胃肠杆菌和Wistar大鼠的肠肌肉中的自噬基因表达产生了不同的影响。这项工作是有限的，因为只使用大鼠，并且不涵盖人体组织。分配20只雄性Wistar大鼠四组：久坐病毒控制，低强度（Li，10米/分钟），中等强度（Mi，20米/分钟），高强度（Hi，30米/分钟）练习。培训进行30分钟/天，每周5次/周为8周。从腓肠肌和肌肌中萃取RNA和蛋白，然后储存在-80℃。用于自噬基因和蛋白质水平的特异性引物和抗体用于半定量PCR和Western印迹。与对照相比，锻炼减少自噬基因LC3（Li和Mi 0.7折叠，Hi 0.8折，P <0.05）的表达，与对照相比，Soleus肌肉（Li，Mi，Hi 0.8倍，P <0.05）。在另一边，我们观察到P62基因表达在胃肠炎（0.8倍，P <0.05）中降低（0.8倍，P <0.05）肌肉，肌肉肌肉（0.9倍，P <0.05），但在唯一（1.1倍，P＆ 0.05）中增加肌肉嗨。该结果与蛋白质水平的变化一致，表明自噬可以通过不同类型的运动来调节。本研究表明，运动和不同类型的肌纤维的强度效应Wistar大鼠骨骼肌中的自噬基因表达。 MI练习增加了腓肠肌和肌肉肌肉中的自噬基因表达，但HI运动降低了Wistar大鼠的肌肉肌肉中的自噬基因表达。与腓肠肌肌肉相比，Soleus肌肉更敏感。

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