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首页> 外文期刊>Journal of the Brazilian Chemical Society >Simultaneous Quantification of Plasma Catecholamines and Metanephrines by LC-MS/MS
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Simultaneous Quantification of Plasma Catecholamines and Metanephrines by LC-MS/MS

机译：通过LC-MS / MS同时定量血浆儿茶酚胺和Metanephines

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The quantification of the low levels of catecholamines and metanephrines in biological fluids is important for clinical screening of pheochromocytoma/paraganglioma and diagnosis of overtraining syndrome in athletes. We introduce a novel, accurate and sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for simultaneous quantification of these biogenic amines in human plasma. Simple protein precipitation combined with rapid 2-aminoethyl diphenylborinate-assisted liquid-liquid extraction allow us to quantify catecholamines and metanephrines over broad concentration ranges. Target analytes were monitored in positive electrospray ionization mode by multiple reaction monitoring. Method performance was validated for linearity, lower limit of quantification, limit of detection, intra-day and inter-assay precision, carry-over, recovery, and matrix effect. The assay was linear within analytical range 25-1000 pg mL-1 for epinephrine, 30-2500 pg mL-1 for norepinephrine, 15-1000 pg mL-1 for dopamine, 25-2000 pg mL-1 for metanephrine and 50-10000 pg mL-1 for normetanephrine, with lower limits of quantification of 15, 20, 10, 15 and 30 pg mL-1, respectively. The intra- and inter-day precisions for all compounds ranged from 0.4 to 6.9% and from 0.9 to 6.6%, respectively. The efficiency of novel method was confirmed by assaying external quality control samples which demonstrated consistent and accurate results.
机译：在生物流体中的低水平儿茶酚胺和Metanephines的定量对于嗜铬细胞瘤/肺炎的临床筛查以及运动员过剩综合征的诊断是重要的。我们介绍一种新型，准确和敏感的液相色谱 - 串联质谱（LC-MS / MS）方法，用于同时定量这些生物胺在人血浆中。简单的蛋白质沉淀与快速的2-氨基乙基二苯基硼酸盐辅助液 - 液萃取允许我们量化CaTecholamines和Metanephrines在广泛的浓度范围内。通过多重反应监测在正电喷雾电离模式下监测靶分析物。方法性能被验证用于线性，量化下限，检测限，日期限制，日期和测定间精度，随身携带，恢复和矩阵效应。该测定在分析范围内为25-1000pg ml-1的线性，用于肾上腺素30-2500pg ml-1，用于多巴胺的15-1000pg ml-1，25-2000pg ml-1用于metanephrine和50-10000对于Narmetanephrine的PG ML-1，分别具有15,20,15和30pg ML-1的较低限制。所有化合物的内部和日间矫反比分别为0.4至6.9％和0.9％至6.6％。通过测定外部质量控制样品来确认新方法的效率，该样品证明一致和准确的结果。

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