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首页> 外文期刊>Journal of Transfusion >Frequencies and Specificities of “Enzyme-Only” Detected Erythrocyte Alloantibodies in Patients Hospitalized in Austria: Is an Enzyme Test Required for Routine Red Blood Cell Antibody Screening?
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Frequencies and Specificities of “Enzyme-Only” Detected Erythrocyte Alloantibodies in Patients Hospitalized in Austria: Is an Enzyme Test Required for Routine Red Blood Cell Antibody Screening?

机译：在奥地利住院患者的患者中检测到“仅酶”的频率和特异性检测到的红细胞含量：是常规红细胞抗体筛选所需的酶测试？

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The aim of this study was to determine the frequencies and specificities of “enzyme-only” detected red blood cell (RBC) alloantibodies in the routine antibody screening and antibody identification in patients hospitalized in Austria. Routine blood samples of 2420 patients were investigated. The antibody screening was performed with a 3-cell panel in the low-ionic strength saline- (LISS-) indirect antiglobulin test (IAT) and with an enzyme-pretreated (papain) 3-cell panel fully automated on the ORTHO AutoVue Innova System. The antibody identification was carried out manually with an 11-cell panel in the LISS-IAT and with an enzyme-pretreated (papain) 11-cell panel. In total 4.05% () of all patients () had a positive RBC antibody screening result. Of them 25.51% (25/98) showed “enzyme-only” detected specific or nonspecific RBC alloantibodies. Rhesus and Lewis system antibodies were found the only specificities of “enzyme-only” RBC alloantibodies all in all 4.8% (4/98) were detected with anti-E, 3.06% (3/98) with anti-, 3.06% (3/98) with anti-D after anti-D prophylaxis and 1.02% (1/98) with anti-e. In total, 14.29% (14/98) showed a nonspecific RBC alloantibody result with the enzyme test. The results of the present study demonstrate that a high number of unwanted positive reactions with the enzyme technique overshadows the detection of “enzyme-only” RBC alloantibodies. (Trial Registration K-37-13).
机译：本研究的目的是确定在在奥地利住院患者常规抗体筛选和抗体鉴定的“酶仅”检测的红血细胞（RBC）的同种抗体的频率和特异性。 2420名患者的常规血液样本进行了调查。用在低离子强度生理盐水（LISS-）间接抗球蛋白测试（IAT）的3电池板进行抗体筛选和用酶预处理（木瓜蛋白酶）3芯面板上的ORTHO AutoVue的伊诺系统完全自动化。该抗体识别与在LISS-IAT和用酶预处理（木瓜蛋白酶）11电池板的11电池板手动地进行。在所有患者中共有4.05％（）（）产生了积极的红细胞抗体筛查结果。它们中的25.51％（98分之25）显示“酶仅”检测的特异性或非特异性RBC同种抗体。恒河猴和Lewis系统中的抗体被发现的“酶仅” RBC同种总而言之4.8％的唯一的特异性（4/98）用抗-E中检测到，3.06％（3/98）用抗，3.06％（3 / 98）用抗E抗d预防后抗d和1.02％（1/98）。总体上，14.29％（14/98）显示与酶测试非特异性同种抗体RBC结果。本研究的结果表明，高数量的与酶技术不需要的阳性反应的掩盖“酶仅” RBC同种抗体的检测。 （试验注册K-37-13）。

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