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首页> 外文期刊>Journal of Translational Medicine >N-myristoyltransferase: A potential novel diagnostic marker for colon cancer
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N-myristoyltransferase: A potential novel diagnostic marker for colon cancer

机译：n-myristoyltransferase：用于结肠癌的潜在新型诊断标志物

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Background Colon cancer is the second leading cause of cancer deaths in the western world. If detected early, colorectal cancer is one of the most treatable forms of cancer. Unfortunately, very few people are screened. N-myristoyltransferase (NMT) catalyzes myristoylation of various proteins including oncoproteins. We have demonstrated earlier the alteration of NMT activity during the progression of colorectal cancer and established NMT as a putative therapeutic target for cancer. Methods Peripheral blood samples and bone marrow were collected from the colon cancer patients and azoxymethane induced colonic tumor rats and their controls respectively. NMT activity and expression was determined as reported earlier. Immunohistochemical studies were carried out using standard procedures. Results In this study we demonstrate for the first time altered expression and localization of NMT in the peripheral blood and bone marrow in colon cancer patients. Immunohistochemical analysis revealed weak to negative staining for NMT in peripheral blood mononuclear cells (PBMC) of controls, whereas strong positivity was observed in PBMC colon cancer patients. In addition, we observed that NMT was localized mostly in the nuclei of the bone marrow (BM) mononuclear cells of the colon cancer patients, whereas NMT remained cytoplasmic in the control bone marrow specimens. Conclusion The strikingly different NMT expression offers the basis of a potential adjunct investigative tool for screening or diagnosis of patients at risk for or suspected of having colon cancer. Furthermore, altered localization of NMT in BM of tumor bearing hosts may serve as an added investigative tool for the diagnostic purpose.
机译：背景技术结肠癌是西方癌症死因的第二个主要原因。如果早期检测到，结直肠癌是最具可治疗的癌症形式之一。不幸的是，很少有人被筛查。 n-myristoyltransferase（nmt）催化各种蛋白质的MyRistoylation，包括癌蛋白。我们曾经证实过结直肠癌进展过程中NMT活性的改变，并将NMT作为癌症的推定治疗靶标。方法分别从结肠癌患者和氮氧基甲烷诱导的结肠癌及其对照中收集外周血样品和骨髓。如前所述确定NMT活性和表达。使用标准程序进行免疫组化研究。结果在本研究中，我们首次证明了结肠癌患者外周血和骨髓中NMT的表达和定位。免疫组织化学分析显示对对照的外周血单核细胞（PBMC）中NMT的阴性染色弱，而PBMC结肠癌患者观察到强阳性。此外，我们观察到NMT主要是在结肠癌患者的骨髓（BM）单核细胞的核中定位，而NMT仍然是对照骨髓标本中的细胞质。结论尖尖不同的NMT表达提供了潜在的辅助调查工具的基础，用于筛查或诊断因患有结肠癌的风险或疑似患者的患者。此外，肿瘤轴承宿主的BM中NMT的改变定位可以作为诊断目的的添加调查工具。

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