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首页> 外文期刊>Journal of Thoracic Disease >Comparison of the next-generation sequencing (NGS) technology with culture methods in the diagnosis of bacterial and fungal infections
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Comparison of the next-generation sequencing (NGS) technology with culture methods in the diagnosis of bacterial and fungal infections

机译：在细菌和真菌感染的诊断中与培养方法的下一代测序（NGS）技术的比较

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Background: Bacterial and fungal infections that caused by various kinds of pathogens are frequently-occurring disease all over the world. To overcome the shortcomings of traditional culture method, we have adapted next-generation sequencing (NGS) technology to identify pathogens. Methods: In this study, clinical samples from 20 patients pre-diagnosed with bacterial and fungal infections in the Shanghai Pulmonary Hospital of Tongji University, China, were investigated retrospectively to compare the NGS with conventional “gold standard” culture methods. Results: The detection rates of bacterial or fungal infections were 95.0% (19/20) by NGS and 60.0% (12/20) by culture method. There was a significant difference between the results of NGS and traditional culture method by using McNemar’s χ2 test (P=0.008). Conclusions: NGS, as an emerging diagnostic technology, shows outstanding advantages in the diagnosis of bacterial and fungal infections, and optimizes the treatment of infectious diseases. The clinical application and future development of NGS technology are worthy of expectation.
机译：背景：各种病原体引起的细菌和真菌感染是世界各地的常见疾病。为了克服传统培养方法的缺点，我们改善了下一代测序（NGS）技术来鉴定病原体。方法：在本研究中，回顾性地调查了中国同济大学上海肺医院预先诊断出细菌和真菌感染的20例患者的临床样本，以比较与传统的“黄金标准”培养方法进行比较NGS。结果：通过NGS和60.0％（12/20）的细菌或真菌感染的检测率为95.0％（19/20），通过培养方法。使用McNemar的χ2检验，NGS和传统培养方法的结果有显着差异（P = 0.008）。结论：作为新兴诊断技术，NGS在诊断细菌和真菌感染方面表现出突出的优势，并优化了传染病的治疗方法。 NGS技术的临床应用和未来发展是值得期望的。

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