...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Medical Microbiology: An Official Journal of the Pathological Society of Great Britain and Ireland >Genetic diversity of influenza A viruses circulating in Bulgaria during the 2018–2019 winter season
【24h】

Genetic diversity of influenza A viruses circulating in Bulgaria during the 2018–2019 winter season

机译：流感的遗传多样性在2018 - 2019年度冬季保加利亚流通的病毒

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Introduction Influenza viruses evolve rapidly and change their antigenic characteristics, necessitating biannual updates of flu vaccines. Aim The aim of this study was to characterize influenza viruses circulating in Bulgaria during the 2018/2019 season and to identify amino acid substitutions in them that might impact vaccine effectiveness. Methodology Typing/subtyping of influenza viruses were performed using real-time Reverse Transcription-PCR (RT-PCR) and results of phylogenetic and amino acid sequence analyses of influenza strains are presented. Results A(H1N1)pdm09 (66?%) predominated over A(H3N2) (34?%) viruses, with undetected circulation of B viruses in the 2018/2019 season. All A(H1N1)pdm09 viruses studied fell into the recently designated 6B.1A subclade with over 50?% falling in four subgroups: 6B.1A2, 6B.1A5, 6B.1A6 and 6B.1A7. Analysed A(H3N2) viruses belonged to subclades 3C.2a1b and 3C.2a2. Amino acid sequence analysis of 36 A(H1N1)pdm09 isolates revealed the presence of six–ten substitutions in haemagglutinin (HA), compared to the A/Michigan/45/2015 vaccine virus, three of which occurred in antigenic sites Sa and Cb, together with four–nine changes at positions in neuraminidase (NA), and a number of substitutions in internal proteins. HA1 D222N substitution, associated with increased virulence, was identified in two A(H1N1)pdm09 viruses. Despite the presence of several amino acid substitutions, A(H1N1)pdm09 viruses remained antigenically similar to the vaccine virus. The 28 A(H3N2) viruses characterized carried substitutions in HA, including some in antigenic sites A, B, C and E, in NA and internal protein sequences. Conclusion The results of this study showed the genetic diversity of circulating influenza viruses and the need for continuous antigenic and molecular surveillance.
机译：引言流感病毒迅速发展，改变了它们的抗原特征，需要进行流感疫苗的两年步更新。目的本研究的目的是在2018/2019年季节中表征在保加利亚循环的流感病毒，并鉴定可能影响疫苗效果的氨基酸取代。使用实时逆转录PCR（RT-PCR）进行方法键入/亚型流感病毒，并介绍了流感菌株的系统发育和氨基酸序列分析的结果。结果A（H1N1）PDM09（66≤％）以（H3N2）（34℃）（34倍）病毒，在2018/2019赛季中的未检测到的B病毒循环。所有（H1N1）PDM09病毒研究落入最近指定的6B.1A亚余亚，其中四个亚组超过50？％：6B.1A2,6B.1A5,6B.1A6和6B.1A7。分析了（H3N2）病毒属于亚克3C.2A1B和3C.2A2。 36A（H1N1）PDM09分离物的氨基酸序列分析显示，与A / MICHIGAN / 45/205疫苗病毒相比，其中三种发生在抗原位点SA和CB中的疫苗病毒，与神经氨酸酶（NA）的阵地的四九变化一起，以及内部蛋白质中的许多取代。 HA1 D222N替代与毒力增加相关，在两种（H1N1）PDM09病毒中鉴定出来。尽管存在几种氨基酸取代，但是（H1N1）PDM09病毒保持抗原地类似于疫苗病毒。该28A（H3N2）病毒表征了HA中的携带取代，包括在Na和内蛋白序列中的抗原位点A，B，C和E中的一些。结论本研究的结果表明循环流感病毒的遗传多样性以及对连续抗原和分子监测的需求。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号