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首页> 外文期刊>BMC Cancer >Novel methods for in vitro modeling of pancreatic cancer reveal important aspects for successful primary cell culture
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Novel methods for in vitro modeling of pancreatic cancer reveal important aspects for successful primary cell culture

机译：胰腺癌体外建模的新方法揭示了原发性细胞培养的重要方面

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BACKGROUND:Pancreatic cancer remains a fatal disease. Experimental systems are needed for personalized treatment strategies, drug testing and to further understand tumor biology. Cell cultures can serve as an excellent preclinical platform, but their generation remains challenging.METHODS:Tumor cells from surgically removed pancreatic ductal adenocarcinoma (PDAC) specimens were cultured under novel protocols. Cellular growth and composition were analyzed and culture conditions were continuously optimized. Characterization of cell cultures and primary tumors was performed via hematoxylin and eosin (HE) and immunofluorescence (IF) staining.RESULTS:Protocols for two- and three-dimensional PDAC primary cell cultures could successfully be established. Primary cell culture depended on dissociation techniques, growth factor supplementation and extracellular matrix components containing Matrigel being crucial for the transformation to three-dimensional PDAC organoids. The generated cultures showed to be highly resemblant to established PDAC primary cell cultures. HE and IF staining for cell culture and corresponding primary tumor characterization could successfully be performed.CONCLUSIONS:The work presented herein shows novel and effective methods to successfully establish primary PDAC cell cultures in a distinct time frame. Factors contributing to cell growth and differentiation could be identified with important implications for further primary cell culture protocols. The established protocols might serve as novel tools in personalized tumor therapy.
机译：背景：胰腺癌仍然是致命的疾病。需要实验系统进行个性化治疗策略，药物检测和进一步了解肿瘤生物学。细胞培养物可以作为优异的临床前平台，但它们的一代仍然存在挑战。方法：来自手术移除的胰腺导管腺癌（PDAC）标本的肿瘤细胞在新颖的方案下培养。分析细胞生长和组合物，培养条件连续优化。通过血毒素和曙红（HE）和免疫荧光（IF）染色来进行细胞培养物和原发性肿瘤的表征。结果：可以成功建立两种和三维PDAC原发性细胞培养物的方案。主要细胞培养依赖于解离技术，含有基质胶的生长因子补充和细胞外基质组分对三维PDAC有机体的转化至关重要。产生的培养物表现出高度同样的成立的PDAC原发性细胞培养物。他和如果可以成功地进行细胞培养和相应的原发性肿瘤表征的染色。结论：本文所述的作品显示了在不同的时间框架中成功建立初级PDAC细胞培养物的新颖和有效方法。有助于细胞生长和分化的因素可以针对进一步的原发性细胞培养方案鉴定出具有重要意义。既定的协议可以作为个性化肿瘤治疗的新型工具。

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