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首页> 外文期刊>BMC Genomics >Comparative expression analysis identifies the respiratory transition-related miRNAs and their target genes in tissues of metamorphosing Chinese giant salamander ( Andrias davidianus )
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Comparative expression analysis identifies the respiratory transition-related miRNAs and their target genes in tissues of metamorphosing Chinese giant salamander ( Andrias davidianus )

机译：比较表达分析鉴定了呼吸道过渡相关的miRNA及其在变型中国巨型蝾螈组织中的靶基因（Andrias Davidianus）

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Chinese giant salamander (Andrias davidianus) undergoes a metamorphosis from aquatic larvae to terrestrial adults, with concomitant transfer of respiration from gills to lungs prior to metamorphosis. These two tissues, as well as skin, were sampled to identify the differentially expressed miRNAs. High-coverage reference transcriptome was generated from combined gill, lung and skin tissues of metamorphosing juveniles, and lung tissue of adults: 86,282 unigenes with total length of approximately 77,275,634?bp and N50 of 1732?bp were obtained. Among these, 13,246 unigenes were assigned to 288 pathways. To determine the possible involvement of miRNAs in the respiratory transition, small RNA libraries were sequenced; 282 miRNAs were identified, 65 among which were known and 217 novel. Based on the hierarchical clustering analysis, the twelve studied samples were classified into three major clusters using differentially expressed miRNAs. We have validated ten differentially expressed miRNAs and some of their related target genes using qPCR. These results largely corroborated the results of transcriptomic and miRNA analyses. Finally, an miRNA-gene-network was constructed. Among them, two miRNAs with target genes related to oxygen sensing were differentially expressed between gill and lung tissues. Three miRNAs were differentially expressed between the lungs of larvae and lungs of adults. This study provides the first large-scale miRNA expression profile overview during the respiration transition from gills to lungs in Chinese giant salamander. Five differentially expressed miRNAs and their target genes were identified among skin, gill and lung tissues. These results suggest that miRNA profiles in respiratory tissues play an important role in the regulation of respiratory transition.
机译：中国巨型蝾螈（Andrias Davidianus）经历了水生幼虫到陆地成年人的变态，伴随着在变态之前从鳃到肺的呼吸转移。将这两种组织以及皮肤被取样以鉴定差异表达的miRNA。高覆盖参考转录组是由变形幼年的组合鳃，肺和皮肤组织产生的，以及成人的肺组织：86,282个ungigenes，总长度约为77,275,634〜Bp和N50的1732磅Bp。其中，将13,246个unigenes分配到288个途径。为了确定miRNA在呼吸转变中的可能累录，测序小RNA文库;鉴定了282名miRNA，其中65名是已知的，217个新颖。基于分层聚类分析，使用差异表达的miRNA分为三个研究样本分为三个主要簇。我们使用QPCR验证了十种差异表达的miRNA和一些相关的靶基因。这些结果在很大程度上证实了转录组和miRNA分析的结果。最后，构建了miRNA-基因网络。其中，两种与氧传感相关的靶基因的miRNA在鳃和肺组织之间差异表达。在幼虫和成人肺的肺部之间差异表达了三个miRNA。本研究提供了在中国巨型蝾螈的鳃转变为肺部的呼吸过渡期间的第一个大规模miRNA表达概述。在皮肤，鳃和肺组织中鉴定了五种差异表达的miRNA及其靶基因。这些结果表明，呼吸组织中的miRNA谱在呼吸过渡调节中发挥着重要作用。

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