...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>BMC Genomics >A-GAME: improving the assembly of pooled functional metagenomics sequence data
【24h】

A-GAME: improving the assembly of pooled functional metagenomics sequence data

机译：A-Game：改进汇集功能组织序列数据的组装

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Expression screening of environmental DNA (eDNA) libraries is a popular approach for the identification and characterization of novel microbial enzymes with promising biotechnological properties. In such “functional metagenomics” experiments, inserts, selected on the basis of activity assays, are sequenced with high throughput sequencing technologies. Assembly is followed by gene prediction, annotation and identification of candidate genes that are subsequently evaluated for biotechnological applications. Here we present A-GAME (A GAlaxy suite for functional MEtagenomics), a web service incorporating state of the art tools and workflows for the analysis of eDNA sequence data. We illustrate the potential of A-GAME workflows using real functional metagenomics data, showing that they outperform alternative metagenomics assemblers. Dedicated tools available in A-GAME allow efficient analysis of pooled libraries and rapid identification of candidate genes, reducing sequencing costs and saving the need for laborious manual annotation. In conclusion, we believe A-GAME will constitute a valuable resource for the functional metagenomics community. A-GAME is publicly available at http://beaconlab.it/agame
机译：环境DNA（EDNA）文库的表达筛查是一种常识和表征具有有前途的生物技术特性的新型微生物酶的普遍方法。在这种“功能性偏心神经学”实验中，基于活性测定选择的插入物用高通量测序技术进行测序。随后是对生物技术应用的基因预测，注释和鉴定随后评估的候选基因的鉴别基因。在这里，我们提出了一个游戏（用于功能性偏心神经的Galaxy Suite），该服务包括用于分析EDNA序列数据的艺术工具和工作流程的Web服务。我们说明了使用真正的功能偏心组织数据的游戏工作流程的潜力，表明它们优于替代的偏见组件组件。 A-Game中提供的专用工具允许有效地分析汇集库和候选基因的快速识别，降低测序成本并节省对费用手动注释的需求。总之，我们认为A-Game将构成功能性偏见群落的宝贵资源。 A-Game公开提供http://beaconlab.it/agame

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号