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首页> 外文期刊>BMC Genomics >Identification of putative fecundity-related gustatory receptor genes in the brown planthopper Nilaparvata lugens
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Identification of putative fecundity-related gustatory receptor genes in the brown planthopper Nilaparvata lugens

机译：棕色Planthopper Nileaparvata Lugens识别推定的繁殖力相关味觉受体基因

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The insect gustatory system plays a central role in the regulation of multiple physiological behaviors and the co-evolution between insects and their hosts. The gustatory receptors (Gr) are important to allow insects to sense their environment. It is critical to the selection of foods, mates and oviposition sites of insects. In this study, the Gr family genes of the brown planthopper (BPH) Nilaparvata lugens St?l (Hemiptera: Delphacidae) were identified and analyzed, and their potential relationship to the fecundity of BPH was explored by RNA interference (RNAi). We identified 32 putative Gr genes by analyzing transcriptome and genome data from BPH. Most of these Gr proteins have the typical structure of seven transmembrane domains. The BPH Gr genes (NlGrs) were expressed in virtually all tissues and stages, whilst higher transcript accumulations were found in adult stages and in the midguts of females. Based on the phylogenic analysis, we classified NlGrs into five potential categories, including 2 sugar receptors, 2 Gr43a-like receptors, 7 CO2 receptors, 5 bitter receptors and 13 NlGrs with unknown functions. Moreover, we found that 10 NlGrs have at least two alternative splicing variants, and obtained alternative splicing isoforms of 5 NlGrs. Finally, RNAi of 29 NlGrs showed that 27 of them are related to the transcript levels of two fecundity related genes vitellogenin and vitellogenin receptor. We found 32 Gr genes in BPH, among which at least 27 are required for normal expression of fecundity markers of this insect pest. These findings provide the basis for the functional study of Grs and the exploration of potential genes involved in the monophagous character of BPH.
机译：昆虫料理系统在调节多种生理行为和昆虫与其主体之间的共同演变中起着核心作用。味肠受体（GR）对于允许昆虫来感受到他们的环境很重要。这对于选择食物，伴侣和昆虫产卵位点至关重要。在这项研究中，鉴定并分析了棕色Planthopper（BPH）NileaParvata Lugens STα1（Hemiptera：Delphacidae）的GR家族基因，并通过RNA干扰（RNAi）探索了与BPH的繁殖力的潜在关系。通过分析来自BPH的转录组和基因组数据，我们鉴定了32个推定的GR基因。大多数这些GR蛋白质具有七个跨膜结构域的典型结构。 BPH GR基因（NLGR）几乎表达了所有组织和阶段，而在成人阶段和女性中肠道中发现了更高的转录物累积。基于系统发生分析，我们将NLGR分为五种潜在类别，包括2种糖受体，2 GR43A样受体，7只CO 2受体，5个苦受体和13个NLGR，具有未知的功能。此外，我们发现10个NLGR具有至少两个替代的剪接变体，并获得了5个NLGR的替代剪接同种型。最后，29个NLGR的RNAi显示，其中27种与两种繁殖会相关基因vallogenin和Vitellogenin受体的转录水平有关。我们在BPH中发现了32克基因，其中至少27例需要这种昆虫害虫的繁殖力标记的正常表达。这些发现提供了GRS功能研究的基础和潜在基因的探索，所述潜在基因参与BPH的直噬细胞。

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