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首页> 外文期刊>International Journal of Molecular Sciences >Melatonin Priming Alleviates Aging-Induced Germination Inhibition by Regulating β-oxidation, Protein Translation, and Antioxidant Metabolism in Oat ( Avena sativa L.) Seeds
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Melatonin Priming Alleviates Aging-Induced Germination Inhibition by Regulating β-oxidation, Protein Translation, and Antioxidant Metabolism in Oat ( Avena sativa L.) Seeds

机译：褪黑激素引发通过调节β-氧化，蛋白翻译和燕麦（Avena Sativa L.）种子的抗氧化代谢来减轻衰老诱导的萌发抑制

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Although melatonin has been reported to play an important role in regulating metabolic events under adverse stresses, its underlying mechanisms on germination in aged seeds remain unclear. This study was conducted to investigate the effect of melatonin priming (MP) on embryos of aged oat seeds in relation to germination, ultrastructural changes, antioxidant responses, and protein profiles. Proteomic analysis revealed, in total, 402 differentially expressed proteins (DEPs) in normal, aged, and aged + MP embryos. The downregulated DEPs in aged embryos were enriched in sucrose metabolism, glycolysis, β-oxidation of lipid, and protein synthesis. MP (200 μM) turned four downregulated DEPs into upregulated DEPs, among which, especially 3-ketoacyl-CoA thiolase-like protein (KATLP) involved in the β-oxidation pathway played a key role in maintaining TCA cycle stability and providing more energy for protein translation. Furthermore, it was found that MP enhanced antioxidant capacity in the ascorbate-glutathione (AsA-GSH) system, declined reactive oxygen species (ROS), and improved cell ultrastructure. These results indicated that the impaired germination and seedling growth of aged seeds could be rescued to a certain level by melatonin, predominantly depending on β-oxidation, protein translation, and antioxidant protection of AsA-GSH. This work reveals new insights into melatonin-mediated mechanisms from protein profiles that occur in embryos of oat seeds processed by both aging and priming.
机译：虽然据报道，褪黑激素在对不良应力下调节代谢事件中发挥重要作用，但其老化种子萌发的潜在机制仍不明确。进行该研究以研究褪黑素引发（MP）对萌发，超微结构变化，抗氧化反应和蛋白质谱的末年燕麦种子胚胎的影响。蛋白质组学分析在正常，老化和老化+ MP胚胎中揭示了总共402种差异表达的蛋白质（DEP）。老化胚胎中的下调的DEP富含蔗糖代谢，糖酵解，脂质的β-氧化和蛋白质合成。 MP（200μm）将四个下调的DEP分为上调的DEP，其中尤其是β-氧化途径中涉及的3-酮酰基-COA硫醇酶样蛋白（KATLP）在维持TCA循环稳定性并为其提供更多能量方面发挥了关键作用蛋白翻译。此外，发现MP增强抗坏血酸谷胱甘肽（ASA-GSH）系统中的抗氧化能力，下降的活性氧物质（ROS）和改善的细胞超超微结构。这些结果表明，通过褪黑素可以救出老化种子的萌发和幼苗生长，主要取决于β-氧化，蛋白翻译和ASA-GSH的抗氧化保护。这项工作揭示了通过两种衰老和引发处理的燕麦种子胚胎中发生的蛋白质谱的新见解。

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