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RT-qPCR Testing of SARS-CoV-2: A Primer

机译:SARS-COV-2的RT-QPCR测试:底漆

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摘要

Testing for the presence of coronavirus is an essential diagnostic tool for monitoring and managing the current COVID-19 pandemic. The only reliable test in current use for testing acute infection targets the genome of SARS-CoV-2, and the most widely used method is quantitative fluorescence-based reverse transcription polymerase chain reaction (RT-qPCR). Despite its ubiquity, there is a significant amount of uncertainty about how this test works, potential throughput and reliability. This has resulted in widespread misrepresentation of the problems faced using this test during the current COVID-19 epidemic. This primer provides simple, straightforward and impartial information about RT-qPCR.
机译:测试冠状病毒的存在是监测和管理当前Covid-19大流行的必要诊断工具。目前用于测试急性感染的唯一可靠测试靶向SARS-COV-2的基因组,最广泛使用的方法是定量荧光的逆转录聚合酶链反应(RT-QPCR)。尽管有笨拙,但是关于该测试的工作,潜在的产量和可靠性有很大的不确定性。这导致了在当前Covid-19流行病中使用该测试面临的问题的广泛歪曲。该引物提供关于RT-QPCR的简单,简单和公正的信息。

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