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首页> 外文期刊>International Journal of Molecular Sciences >Collagen/Chitosan Functionalization of Complex 3D Structures Fabricated by Laser Direct Writing via Two-Photon Polymerization for Enhanced Osteogenesis
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Collagen/Chitosan Functionalization of Complex 3D Structures Fabricated by Laser Direct Writing via Two-Photon Polymerization for Enhanced Osteogenesis

机译：通过双光子聚合通过双光子聚合制备的复合3D结构的胶原蛋白/壳聚糖官能化，用于增强成骨发生

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摘要

The fabrication of 3D microstructures is under continuous development for engineering bone substitutes. Collagen/chitosan (Col/CT) blends emerge as biomaterials that meet the mechanical and biological requirements associated with bone tissue. In this work, we optimize the osteogenic effect of 3D microstructures by their functionalization with Col/CT blends with different blending ratios. The structures were fabricated by laser direct writing via two-photons polymerization of IP-L780 photopolymer. They comprised of hexagonal and ellipsoidal units 80 μm in length, 40 μm in width and 14 μm height, separated by 20 μm pillars. Structures’ functionalization was achieved via dip coating in Col/CT blends with specific blending ratios. The osteogenic role of Col/CT functionalization of the 3D structures was confirmed by biological assays concerning the expression of alkaline phosphatase (ALP) and osteocalcin secretion as osteogenic markers and Alizarin Red (AR) as dye for mineral deposits in osteoblast-like cells seeded on the structures. The structures having ellipsoidal units showed the best results, but the trends were similar for both ellipsoidal and hexagonal units. The strongest osteogenic effect was obtained for Col/CT blending ratio of 20/80, as demonstrated by the highest ALP activity, osteocalcin secretion and AR staining intensity in the seeded cells compared to all the other samples.
机译：3D微观结构的制造是在持续发育工程骨替代品。胶原蛋白/壳聚糖（Col / CT）融合为符合与骨组织相关的机械和生物学要求的生物材料。在这项工作中，我们通过用不同混合比用Col / Ct混合物的功能化优化3D微观结构的成骨效果。通过IP-L780光聚合物的双光子聚合通过激光直接写入来制造该结构。它们由长度为80μm的六边形和椭圆形单元，宽度为40μm，高度为14μm，分离为20μm柱。通过具有特异性混合比的Col / CT共混物中的浸涂来实现结构的官能化。通过关于碱性磷酸酶（ALP）和骨钙素分泌作为成骨状标记和茜素红（AR）作为矿沉积物的染料，通过生物学测定来证实3D结构的骨质发生作用。结构。具有椭圆形单元的结构显示出最佳结果，但椭圆形和六边形单元的趋势相似。对于Col / Ct混合比率为20/80的Col / Ct混合比获得最强的成骨效果，如最高ALP活性，骨癌分泌和接种细胞中的AR染色强度与所有其他样品相比所证明的。

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