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首页> 外文期刊>International Journal of Molecular Sciences >BcMF30a and BcMF30c, Two Novel Non-Tandem CCCH Zinc-Finger Proteins, Function in Pollen Development and Pollen Germination in Brassica campestris ssp. chinensis
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BcMF30a and BcMF30c, Two Novel Non-Tandem CCCH Zinc-Finger Proteins, Function in Pollen Development and Pollen Germination in Brassica campestris ssp. chinensis

机译：BCMF30A和BCMF30C，两种新型非串联CCCH锌手指蛋白，在甘蓝型Campestris SSP中的花粉发育和花粉萌发。中国人

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摘要

Chinese cabbage ( Brassica campestris ) is an economically important leaf vegetable crop worldwide. Mounting studies have shown that cysteine-cysteine-cysteine-histidine (CCCH) zinc-finger protein genes are involved in various plant growth and development processes. However, research on the involvement of these genes in male reproductive development is still in its infancy. Here, we identified 11 male fertility-related CCCH genes in Chinese cabbage. Among them, a pair of paralogs encoding novel non-tandem CCCH zinc-finger proteins, Brassica campestris Male Fertility 30a ( BcMF30a ) and BcMF30c , were further characterized. They were highly expressed in pollen during microgametogenesis and continued to express in germinated pollen. Further analyses demonstrated that both BcMF30a and BcMF30c may play a dual role as transcription factors and RNA-binding proteins in plant cells. Functional analysis showed that partial bcmf30a bcmf30c pollen grains were aborted due to the degradation of pollen inclusion at the microgametogenesis phase, and the germination rate of viable pollen was also greatly reduced, indicating that BcMF30a and BcMF30c are required for both pollen development and pollen germination. This research provided insights into the function of CCCH proteins in regulating male reproductive development and laid a theoretical basis for hybrid breeding of Chinese cabbage.
机译：大白菜（Brassica Campestris）是全球经济上重要的叶蔬菜作物。安装研究表明，半胱氨酸 - 半胱氨酸 - 半胱氨酸 - 组氨酸（CCCH）锌 - 手指蛋白基因参与各种植物生长和发育过程。然而，关于这些基因在雄性生殖发展中的参与的研究仍在其初期。在这里，我们确定了大白菜中的11名男性生育率相关的CCCH基因。其中，进一步表征了一对编码新型非串联CCCH锌手指蛋白，芸苔Chipestris雄性生育率30a（BCMF30A）和BCMF30C的副蛋白酶。在微酰胺发生期间，它们在花粉中表达，并继续在发芽的花粉中表达。进一步分析证明BCMF30A和BCMF30C可以在植物细胞中发挥双重作用和RNA结合蛋白。功能分析表明，由于在微酰胺发生阶段的花粉夹杂物的降解，部分BCMF30A BCMF30C花粉晶体中止了，可行花粉的发芽率也大大降低，表明花粉发育和花粉萌发需要BCMF30A和BCMF30C。本研究提供了CCCH蛋白在调节男性生殖开发方面的功能的见解，并为大白菜杂交繁殖的理论依据。

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