• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>RSC Advances >A smart fluorescent biosensor for the highly sensitive detection of BRCA1 based on a 3D DNA walker and ESDR cascade amplification
【24h】

A smart fluorescent biosensor for the highly sensitive detection of BRCA1 based on a 3D DNA walker and ESDR cascade amplification

机译：基于3D DNA步行者和ESDR级联放大的BRCA1的高灵敏度检测的智能荧光生物传感器

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Nucleic acid analysis plays an important role in the diagnosis of diseases. There is a continuous demand to develop rapid and sensitive methods for the specific detection of nucleic acids. Herein, we constructed a highly sensitive and rapid fluorescent biosensor for the detection of BRCA1 by coupling a 3D DNA walker machine with spontaneous entropy-driven strand displacement reactions (ESDRs). In this study, the 3D DNA walker machine was well activated by the target DNA; this resulted in the cyclic utilization of the target DNA and the release of intermediate DNAs. Subsequently, the free intermediate DNAs triggered the circulation process of ESDRs with the help of the assistant probe A, leading to a significant enhancement of the fluorescence intensity. Due to the robust execution of the 3D DNA walker machine and highly efficient amplification capability of ESDRs, the developed biosensing method shows a wide linear range from 0.1 pM to 10 nM with the detection limit as low as 41.44 fM (S/N = 3). Moreover, the constructed biosensor displays superior specificity and has been applied to monitor BRCA1 in complex matrices. Thus, this elaborated cascade amplification biosensing strategy provides a potential platform for the bioassays of nucleic acids and the clinical diagnosis of diseases.
机译：核酸分析在疾病的诊断中起着重要作用。对于核酸的特异性检测，有一种不断的需求，可以发育快速和敏感的方法。在此，我们通过耦合具有自发熵驱动的链位移反应（ESDRS）的3D DNA Walker机器来检测BRCA1的高敏感和快速的荧光生物传感器。在这项研究中，3D DNA步行者机通过靶DNA激活很好;这导致靶DNA的循环利用率和中间体DNA的释放。随后，自由中间DNA在助理探针A的帮助下引发了ESDR的循环过程，从而显着提高了荧光强度。由于3D DNA步行者机器的稳健执行和ESDR的高效放大能力，所开发的生物传感方法显示宽线性范围从0.1pm至10nm的宽线性范围，检测极限低至41.44 fm（s / n = 3） 。此外，构造的生物传感器显示出优异的特异性，并且已经应用​​于在复杂的基质中监测BRCA1。因此，这种详细的级联扩增生物传感策略为核酸生物测定和疾病的临床诊断提供了潜在的平台。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号