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Exploration of a high-efficiency and low-cost technique for maximizing the glucoamylase production from food waste

机译：高效率和低成本技术的探索，用于最大化食物垃圾的葡糖淀粉酶生产

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This study was aimed at the exploration of high-efficiency and low-cost technique for glucoamylase (GA) production from food waste; moreover, the produced GA could be directly used in the hydrolysis of food waste. A mixture of food waste, rice waste and cake waste as a sole feedstock was investigated for the production of GA via solid-state fermentation. The highest GA activity of 458.3 U g ~(?1) was obtained from the rice waste after 9 days of incubation. The cake waste also demonstrated a high GA production, achieving 406.5 U g ~(?1) dry substrate. However, the most practical substrate for GA production that could be integrated in the food waste treatment was the mixed food waste, which could effectively produce GA without any additives or adjustments using the technique developed in this study. The optimum conditions for GA production from the mixed food waste were determined through a response surface methodology: the temperature of 31.16 °C, the inoculum amount of 1.54 mL, and the time of fermentation of 7.81 days. The maximum GA activity of 180.59 U g ~(?1) could be achieved under these optimum conditions, which was actually much higher than those reported in the literature. This study showed that the mixed food waste could be an ideal feedstock for the on-site production of high-activity GA, and the produced GA could be directly applied in food waste hydrolysis, which significantly reduced the process cost.
机译：本研究旨在探讨食品废物中葡糖和葡糖淀粉酶（GA）生产的高效率和低成本技术;此外，所生产的GA可以直接用于食物废物的水解。研究了食物废物，水稻废料和蛋糕废物作为鞋底原料的混合物，通过固态发酵生产Ga。在孵育9天后，从水稻废物中获得了458.3u g〜（α1）的最高GA活性。蛋糕废物还证明了高GA生产，实现了406.5μg〜（α1）干基质。然而，可以集成在食物废物处理中的GA生产的最实用的基材是混合食品废物，其可以有效地生产没有任何添加剂或使用本研究中开发的技术的添加剂或调整。通过响应面方法测定来自混合食品废料的GA生产的最佳条件：31.16℃的温度，接种物量为1.54mL，发酵时间为7.81天。在这些最佳条件下可以实现180.59u g〜（α1）的最大GA活性，其实际上远高于文献中报告的条件。这项研究表明，混合食品废物可以是高活度Ga的现场生产的理想原料，所生产的GA可以直接应用于食品废物水解，这显着降低了工艺成本。

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