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首页> 外文期刊>RSC Advances >Effect of the inserted active-site-covering lid loop on the catalytic activity of a mutant B. subtilis γ-glutamyltransferase (GGT)
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Effect of the inserted active-site-covering lid loop on the catalytic activity of a mutant B. subtilis γ-glutamyltransferase (GGT)

机译：插入的有效位点覆盖盖环对突变体γ-谷氨酰胺转移酶（GGT）的催化活性的影响

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摘要

γ-Glutamylpeptides are compounds derived from the acylation of an amino acid or a short peptide by the γ-carboxyl carbon of the side chain of glutamic acid. Due to their altered chemico-physical and organoleptic properties, they may be interesting substitutes or precursors of parent compounds used in pharmaceutical, dietetic and cosmetic formulations. Some of them are naturally occurring flavor enhancers or are endowed with biological activities. Enzymatic approaches to the synthesis of γ-glutamyl derivatives based on the use of γ-glutamyltransferases (GGTs, EC 2.3.2.2) have been proposed, which should be able to alleviate the problems connected with the troublesome and low-yielding extraction from natural sources or the non-economical chemical synthesis, which requires protection/deprotection steps. With the aim of overcoming the current limitations in the use of GGTs as biocatalysts, a mutant GGT was investigated. The mutant GGT was obtained by inserting the active-site-covering lid loop of the E. coli GGT onto the structure of B. subtilis GGT. With respect to the wild-type enzyme, the mutant showed a more demanding substrate specificity and a low hydrolase activity. These results represent an attempt to correlate the structural features of a GGT to its different activities. However, the ability of the mutant enzyme to catalyze the subsequent addition of several γ-glutamyl units, inherited by the parent B. subtilis GGT, still represents a limitation to its full application as a biocatalyst for preparative purposes.
机译：γ-谷氨酰肽是通过谷氨酸侧链的γ-羧基碳衍生自氨基酸的酰化或短肽的化合物。由于其改变的化学物理和感官特性，它们可能是药物，饮食和化妆品制剂中使用的母体化合物的有趣替代物或前体。其中一些是天然存在的味道增强剂，或者赋予生物活性。已经提出了基于γ-谷氨酰胺转移酶（GGTS，EC 2.3.2.2）的γ-谷氨酸衍生物合成γ-谷氨酸衍生物的酶促方法，这应该能够缓解与自然来源的麻烦和低产萃取相关的问题或非经济化学合成，需要保护/脱保护步骤。旨在克服使用GGTS作为生物催化剂的当前限制，研究了突变GGT。通过将大肠杆菌GGT的脊髓覆盖环插入B.枯草芽孢杆菌GGT的结构来获得突变GGT。关于野生型酶，突变体显示出更苛刻的底物特异性和低水解酶活性。这些结果表示试图将GGT的结构特征与其不同的活动相关联。然而，突变酶催化由父母B.枯草芽孢杆菌GGT遗传的几种γ-谷氨酸单元的能力仍然是其作为用于制备目的的生物催化剂的完全应用的限制。

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