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One-pot synthesis of biomimetic glutathione peroxidase with temperature responsive catalytic behaviors

机译：具有温度响应性催化行为的仿生谷胱甘肽过氧化物酶的单壶合成

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Excessive reactive oxygen free radicals (ROS) are the main cause of various oxidative diseases. It is of great significance to develop antioxidant drugs that can intelligently regulate free radical concentrations. The biomimetic simulation of glutathione peroxidase (GPx) can provide an important theoretical basis for the development of antioxidant drugs. In order to explore a simple and efficient strategy for constructing biomimetic GPx, a microgel biomimetic GPx ( PNTegel ) with temperature responsive catalytic activity was prepared by a one-pot synthesis method. The PNTegel , with typical enzymatic catalytic characteristics, exhibited a maximum catalytic activity at 37 °C ( υ _(0) = 11.51 mM min ~(?1) ). The investigation of the catalytic mechanism of PNTegel suggested that the binding of different hydrophobic substrates to PNTegel was altered by the change of hydrophobicity of poly( N -isopropylacrylamide) (PNIPAM) in the microgel scaffold of PNTegel during the temperature response process. The change of hydrophobicity was the main factor for regulating the catalytic activity of PNTegel , which resulted in a temperature responsive catalytic behavior of PNTegel . This new strategy for the simple and efficient construction of biomimetic GPx by a one-pot method provides important theoretical support for exploring the preparation of highly effective antioxidant drugs.
机译：过多的反应性氧自由基（ROS）是各种氧化疾病的主要原因。开发可以智能地调节自由基浓度的抗氧化药物具有重要意义。谷胱甘肽过氧化物酶（GPX）的仿真模拟可以为抗氧化药的发育提供重要的理论依据。为了探讨构建生物摩擦GPX的简单有效的策略，通过单罐合成方法制备具有温度响应催化活性的微凝胶生物摩擦GPX（PNTEGEL）。具有典型的酶促催化特性的PNTEGEL在37℃下在37℃（≥（0）= 11.51mm min〜（α1））上显示出最大催化活性。 PNTEGEL催化机制的研究表明，通过在温度响应过程中PNTEGEL微凝胶微凝胶支架中的聚（N-异丙基丙烯酰胺）（PNIPAM）的疏水性改变了不同疏水基材与PNTEGEL的结合改变。疏水性的变化是调节pntegel催化活性的主要因素，这导致pntegel的温度响应性催化行为。通过单锅方法简单高效地建造生物摩擦GPX的这种新策略为探索高效抗氧化药物的制备提供了重要的理论支持。

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