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Mechanical and physicochemical behavior of a 3D hydrogel scaffold during cell growth and proliferation

机译：细胞生长和增殖中3D水凝胶支架的机械和物理化学行为

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Some of the essential properties for cellular scaffolding are the capability to maintain the three-dimensional (3D) structure, good adhesion, and adequate elastic modulus during cell growth, migration, and proliferation. Biocompatible synthetic hydrogels are commonly used as cellular scaffolds because they can mimic the natural extracellular matrices (ECMs). However, it is possible that the physicochemical and mechanical behavior of the scaffold changes during cell proliferation and loses the scaffold properties but this is rarely monitored. In this work, the physicochemical and mechanical properties of a macroporous soft material based on poly( N -isopropyl acrylamide) (PNIPAM) have been studied during a period of 75 days at culture condition while bovine fetal fibroblasts (BFF) were grown within the matrix. The interconnected macroporous hydrogel was obtained by cryogelation at ?18 °C. The swelling capacity of the scaffold was not altered during cell proliferation but changes in the mechanical properties were observed, beginning with the high elastic modulus (280 kPa) that progressively decreased until mechanical stability (40 kPa) was achieved after 20 culture days. It was observed that the matrix–cell interactions together with collagen production favor normal cellular processes such as cell morphology, adhesion, migration, and proliferation. Therefore, the observed behavior of macroporous PNIPAM as a 3D scaffold during cell growth indicates that the soft matrix is cytocompatible for a long time and preserves the suitable properties that can be applied in tissue engineering and regenerative medicine.
机译：细胞支架的一些基本性质是在细胞生长，迁移和增殖期间保持三维（3D）结构，良好的粘合性和适当的弹性模量的能力。生物相容性合成水凝胶通常用作细胞支架，因为它们可以模拟天然细胞外基质（ECMS）。然而，在细胞增殖期间支架的物理化学和机械性能变化并失去支架性质，但很少监测这一点。在这项工作中，在培养条件的75天的时间内研究了基于聚（N-异丙基丙烯酰胺）（PNIPAM）的大孔软材料的物理化学和力学性质，同时在基质中生长牛胎成纤维细胞（BFF） 。通过在α18℃下冷冻胶片获得互连的大孔水凝胶。在细胞增殖期间没有改变支架的膨胀能力，但观察到机械性能的变化，从高弹量模（280kPa）开始，直到20培养天后达到机械稳定性（40kPa）。观察到基质 - 细胞相互作用与胶原蛋白产生有利于正常的细胞过程，例如细胞形态，粘附，迁移和增殖。因此，在细胞生长期间观察到大孔肺蛋白作为3D支架的观察到的行为表明，软质基质长时间是细胞织立性，并保留可用于组织工程和再生药物的合适性质。

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