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首页> 外文期刊>RSC Advances >Graphene oxide-Au nano particle coated quartz crystal microbalance biosensor for the real time analysis of carcinoembryonic antigen
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Graphene oxide-Au nano particle coated quartz crystal microbalance biosensor for the real time analysis of carcinoembryonic antigen

机译：石墨烯氧化物-Au纳米颗粒涂层石英晶微观生物传感器，用于实时分析癌胚抗原

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摘要

A label-free quartz crystal microbalance (QCM) biosensor was developed for the selective and real-time estimation of carcinoembryonic antigen (CEA) through the present study. Graphene oxide-Au nanoparticles (GO-AuNPs) was in situ synthesised on the surface of the QCM electrode and the antibody of CEA (monoclonal anti-CEA from mouse) was covalently immobilized on this layer as the bioreceptor for CEA. Mercaptoacetic acid–EDC–NHS reaction mechanism was used for anti-CEA immobilization. The effect of oxygen plasma treatment of the QCM electrode surface before bioreceptor preparation on the performance of the biosensor was tested and was found promising. CEA solutions with various concentrations were analysed using the bioreceptors to estimate the sensitivity and detection limit of the biosensor. The biosensors selectively recognized and captured CEA biomolecules with a detection limit of 0.06 and 0.09 ng mL ~(?1) of CEA for oxygen plasma-treated (E2) and untreated (E1) bioreceptors, respectively. The sensitivity was estimated at 102 and 79 Hz, respectively, for E2 and E1. Clinical serum samples were analysed and the results were found in good agreement with the ELISA analysis. Long term stability was also found to be excellent. Langmuir adsorption isotherm was also conducted using the experimental results.
机译：通过本研究开发了一种无标签的石英晶体微稳定（QCM）生物传感器，用于Carcinoembryonic抗原（CEA）的选择性和实时估计。石墨烯-Au纳米颗粒（GO-AUNP）在QCM电极的表面上合成，CEA（小鼠单克隆抗CEA）的抗体在该层上共价固定为CEA的生物团体。巯基乙酸-EDC-NHS反应机理用于防CEA固定化。测试了QCM电极表面的氧等离子体处理对生物传感器的性能之前的氧血浆电极表面的影响，并发现了有前景。使用生物团分析具有各种浓度的CEA溶液，以估计生物传感器的灵敏度和检测极限。生物传感器分别选择性地识别和捕获CEA生物分子，其检测限为0.06和0.09 ng mL〜0.09ng〜（α1）的CEA，用于氧等离子体处理（E2）和未处理（E1）生物受体。对于E2和E1，分别在102和79Hz处估计灵敏度。分析了临床血清样品，并与ELISA分析吻合良好的结果。也发现长期稳定性是优异的。还使用实验结果进行朗米尔吸附等温线。

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