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首页> 外文期刊>RSC Advances >Bio-inspired facile fabrication of silver nanoparticles from in vitro grown shoots of Tamarix nilotica: explication of its potential in impeding growth and biofilms of Listeria monocytogenes and assessment of wound healing ability
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Bio-inspired facile fabrication of silver nanoparticles from in vitro grown shoots of Tamarix nilotica: explication of its potential in impeding growth and biofilms of Listeria monocytogenes and assessment of wound healing ability

机译：生物启发性的玉米喹甲尼尔替氏菌的银纳米粒子的生物纳米粒子：潜在潜在潜伏期的潜力和伤口愈合能力的评估潜力

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Novel, safe, and effective antilisterial agents are required in order to prevent Listeria monocytogenes infections and maintain food safety. This study synthesized silver nanoparticles (AgNPs) from the shoot extract of in vitro -grown Tamarix nilotica (TN) and characterized them using X-ray diffraction, Fourier transform infrared spectroscopy, UV-visible spectroscopy, dynamic light scattering, scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDXS), and transmission electron microscopy (TEM). We also assessed the antilisterial potential of the synthesized TN-AgNPs by determining the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) against two strains of L. monocytogenes and L. innocua . TN-AgNPs (2×MICs) showed a significant decrease in growth in all Listeria test strains. Release of cellular content and cell morphology analysis of TN-AgNP-treated bacterial cells demonstrated the mechanism of bactericidal activity of AgNPs. In addition, TN-AgNPs induced a significant decrease in swimming motility (62–71%), biofilm formation (57–64%), and preformed biofilms (48–58%) in all Listeria test strains at sub-inhibitory concentrations. Microtitre plate assay results for biofilm inhibition were confirmed by SEM and CLSM visualization of TN-AgNP-treated and TN-AgNP-untreated Listeria test strains. TN-AgNPs also showed wound-healing activity in MCF-7 cells by inhibiting cell migration in a scratch plate assay. TN-AgNP-induced enhanced reactive oxygen species generation in treated cells could be a plausible reason for the biofilm inhibitory activity of AgNPs. TN-AgNPs having antilisterial, antibiofilm, and wound-healing properties can be effectively used to prevent L. monocytogenes infections in the food industry and healthcare.
机译：需要进行新颖，安全和有效的止止剂，以防止李斯特菌单核细胞增生感染和维持食品安全性。本研究从体外 - 制轧尼龙（TN）的枝条提取物合成银纳米粒子（AgNP），并使用X射线衍射，傅里叶变换红外光谱，UV可见光谱，动态光散射，扫描电子显微镜（SEM ），能量分散X射线光谱（EDX）和透射电子显微镜（TEM）。我们还通过测定用于两种L.单核细胞增生和L.Innocua的两种菌株的最小抑制浓度（MIC）和最小杀菌浓度（MBC）来评估合成的TN-AGNP的止止势电位。 TN-AGNPS（2×MICS）显示出所有李斯特菌试验菌株的生长显着降低。释放细胞含量和TN-AgNP处理细菌细胞的细胞形态分析证明了AgNP的杀菌活性的机制。此外，在亚抑制浓度下，TN-AgNP诱导游泳运动（62-71％），生物膜形成（57-64％），生物膜形成（57-64％）和预先形成的生物膜（48-58％）进行显着降低。通过TN-AgNP处理和TN-AgNP-未处理的李斯特菌试验菌株的SEM和CLSM可视化证实了用于生物膜抑制的微量滴定板测定结果。 TN-AGNP通过抑制刮擦板测定中的细胞迁移，还在MCF-7细胞中显示出伤口愈合活性。治疗细胞中的TN-AgNP诱导的增强的活性氧物质产生可能是生物膜抑制作用agnps的合理原因。可以有效地使用具有止血，抗腹膜和伤口愈合性能的TN-AGNP来预防食品工业和医疗保健中的L.单核细胞增生感染。

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