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首页> 外文期刊>RSC Advances >Extraction and purification of ε-poly-l-lysine from fermentation broth using an ethanol/ammonium sulfate aqueous two-phase system combined with ultrafiltration
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Extraction and purification of ε-poly-l-lysine from fermentation broth using an ethanol/ammonium sulfate aqueous two-phase system combined with ultrafiltration

机译：用乙醇/硫酸铵水性双相体系与超滤的发酵液中ε-聚-L-赖氨酸的提取和纯化

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ε-Poly- L -lysine (ε-PL) serves as a natural food preservative and is manufactured mainly by extraction from microbial fermentation broth using ion-exchange chromatography. In order to develop an alternative purification strategy, an environmentally friendly alcohol/salt aqueous two-phase system (ATPS) was explored in this study for ε-PL extraction. A study of the separation of ε-PL in different alcohol/salt systems showed that ethanol/ammonium sulfate ATPS exhibited the highest ε-PL partition coefficient and recovery ratio. Based on the phase diagram, the effect of phase composition on partition, and the removal of pigment and protein, an ATPS that was composed of 20% (w/w) ethanol and 20% (w/w) ammonium sulfate, with a feedstock at pH 9.5, was developed to extract ε-PL from the fermentation broth. This achieved an ε-PL recovery ratio of 96.15% with an ε-PL purity of 40.23% after triplicate extractions. Subsequently, desalting by ultrafiltration led to a final ε-PL product of 92.39% purity and 87.72% recovery. The ethanol/ammonium sulfate ATPS provides a new possibility for ε-PL purification.
机译：ε-聚溶胶（ε-PL）用作天然食品防腐剂，主要通过离子交换色谱法从微生物发酵液中提取来制造。为了开发替代纯化策略，在该研究中探讨了对环境友好的醇/盐水性两相体系（ATP），用于ε-PL提取。不同醇/盐系统中ε-PL分离的研究表明，乙醇/硫酸铵ATP具有最高的ε-PL分区系数和回收率。基于相图，相成分对分区的影响，以及除去颜料和蛋白质的去除，其由20％（w / w）乙醇组成的ATP和20％（w / w）硫酸铵，用原料在pH9.5开始，开发出从发酵液中提取ε-pl。这实现了ε-PL回收率为96.15％，ε-PL纯度在三次萃取后40.23％。随后，通过超滤的脱盐导致最终ε-PL产物为92.39％纯度和87.72％的回收率。乙醇/硫酸铵ATP提供ε-PL纯化的新可能性。

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