• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>RSC Advances >Sensible graphene oxide differentiates macrophages and Leishmania: a bio-nano interplay in attenuating intracellular parasite
【24h】

Sensible graphene oxide differentiates macrophages and Leishmania: a bio-nano interplay in attenuating intracellular parasite

机译：合理的石墨烯氧化物区分巨噬细胞和莱山米：生物纳米在减少细胞内寄生虫中的相互作用

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Leishmania is an obligate intracellular protozoan parasite, which resides in human macrophage vacuoles that are referred to as parasitophorus vacuoles. Amphotericin B (AmB) is the first-line drug with 99% cure rates; however, overdose-induced toxic side effects are a major limitation. To improve the efficacy at lower dose and subsequently to avoid toxicity and to further investigate the role of charge dynamics on the efficacy, a graphene oxide (GO)-based composite of AmB was developed with native negatively charged GO and amine-conjugated positively charged AGO. The AGO composite resulted in enhanced uptake as confirmed by confocal and FACS analysis. Thus, AGO caused a strong inhibition of amastigotes, with IC _(50) values 5-fold lower than free AmB. The parasitophorus vacuoles harbour a hydrolytic and acidic environment, which is favourable for the parasites, as they don't attenuate this condition. AGO–AmB was able to modify the intracellular pH of the Leishmania donovani -infected macrophages, generating unfavourable conditions for the amastigote, and thus improving its efficacy.
机译：Leishmania是一种迫使细胞内原生动物寄生虫，其存在于人巨噬细胞液泡中称为胰腺炎植物。两性霉素B（AMB）是一排药物，固化率为99％;然而，过量诱导的有毒副作用是一个主要限制。为了提高较低剂量的疗效，随后避免毒性，并进一步研究充电动力学对疗效的作用，并在AMB的基础上进行了基础的氧化石墨烯（GO）的综合，并积极收取胺缀合的胺。以前，Composite导致增强的摄取，通过共聚焦和FACS分析确认。因此，以前引起了对Amastigotes的强烈抑制，IC _（50）值比自由伏夫低5倍。寄生虫液泡含有水解和酸性环境，这对寄生虫有利，因为它们不会衰减这种情况。以前-BAM能够改变Leishmania Donovani-infed巨噬细胞的细胞内pH值，为Amastigotoote产生不利的条件，从而提高其疗效。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号