A polycarboxylic chelating ligand for efficient resin purification of His-tagged proteins expressed in mammalian systems

Codru?a C. Popescu; Marius C. Stoian; Lia-Maria Cucos; Anca G. Coman; Antonio Radoi; Anca Paun; Niculina D. H?dade; Arnaud Gautier; Costin-Ioan Popescu; Mihaela Matache

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A polycarboxylic chelating ligand for efficient resin purification of His-tagged proteins expressed in mammalian systems

机译：一种多羧基螯合配体，用于高效树脂纯化在哺乳动物系统中表达的他标记的蛋白质

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We describe the synthesis of a novel polyamino polycarboxylic ligand, its ability to coordinate metal-ions and attachment to a solid support designed for protein purification through Immobilised Metal-ion Affinity Chromatography (IMAC). The resin was found to be highly efficient for purification of His-tagged HCV E2 glycoproteins expressed in 293T mammalian cells.

机译：我们描述了一种新型多氨基多羧酸配体的合成，其能够通过固定化金属离子亲和层析（IMAC）来协调金属离子和设计成用于蛋白质纯化的固体载体的固体载体。发现树脂是高效的，用于纯化其标记的HCV E2糖蛋白在293T哺乳动物细胞中表达的纯化。

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《RSC Advances》 |2020年第40期|共5页
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Codru?a C. Popescu; Marius C. Stoian; Lia-Maria Cucos; Anca G. Coman; Antonio Radoi; Anca Paun; Niculina D. H?dade; Arnaud Gautier; Costin-Ioan Popescu; Mihaela Matache;
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1. A polycarboxylic chelating ligand for efficient resin purification of His-tagged proteins expressed in mammalian systems [J] . Popescu Codruta C., Stoian Marius C., Cucos Lia-Maria, RSC Advances . 2020,第40期

机译：一种多羧基螯合配体，用于高效树脂纯化在哺乳动物系统中表达的他标记的蛋白质
2. Expression and purification of the recombinant His-tagged GST-CD38 fusion protein using the baculovirus/insect cell expression system [J] . Khoo KM, Chang CF, Schubert J, Protein Expression and Purification . 2005,第2期

机译：使用杆状病毒/昆虫细胞表达系统表达和纯化重组His标记的GST-CD38融合蛋白
3. Efficient purification of selenoprotein thioredoxin reductase 1 by using chelating reagents to protect the affinity resins and rescue the enzyme activities [J] . Sun Shibo, Xu Weiping, Zhou Hao, Process Biochemistry . 2021,第Feba期

机译：使用螯合试剂来保护亲和力树脂并拯救酶活性，有效地纯化硒蛋白硫酸丁蛋白还原酶1
4. NiCo21(DE3): A BL21(DE3) Derivative Designed for Expression and Purification of His-Tagged Recombinant Protein [C] . James C. Satnuelson, Carine Robichon-Iyer, Jianying Luo PEGS . 2015

机译：Nico21（DE3）：BL21（DE3）衍生物，用于表达和纯化他标记的重组蛋白
5. Chelating Ligands and Nanomaterials Based on Graphene Oxide for the Reduction and Sequestration of Radiometals and Protein Purification [D] . Groveman, Sam. 2017

机译：基于石墨烯的螯合配体和纳米材料进行辐射氧化物的减少和封存和蛋白质纯化
6. Heterologous Expression and Purification Systems for Structural Proteomics of Mammalian Membrane Proteins [O] . Isabelle Mus-Veteau 2002

机译：哺乳动物膜蛋白结构蛋白质组学的异源表达和纯化系统
7. A polycarboxylic chelating ligand for efficient resin purification of His-tagged proteins expressed in mammalian systems [O] . Codruţa C. Popescu, Marius C. Stoian, Lia-Maria Cucos, 2020

机译：一种多羧基螯合配体，用于高效树脂纯化在哺乳动物系统中表达的他标记的蛋白质
8. Bacterial-based Systems for Expression and Purification of Recombinant Lassa Virus Proteins of Immunological Relevance; Journal article [R] . Branco, L. M., Matschiner, A., Fair, J. N., 2008

机译：用于表达和纯化免疫相关重组拉沙病毒蛋白的基于细菌的系统;杂志文章

A polycarboxylic chelating ligand for efficient resin purification of His-tagged proteins expressed in mammalian systems

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