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Removal of the A10 adenosine in a DNA-stabilized Ag16 nanocluster

机译:在DNA稳定的AG16纳米光泽颗粒中除去A10腺苷

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The role of the terminal adenosine (A _(10) ) on the spectroscopic and structural properties of a previously described DNA-stabilized Ag _(16) nanocluster (DNA:Ag _(16) NC) is presented. In the original DNA:Ag _(16) NCs (5′-CACCTAGCGA-3′), the A _(10) nucleobase was involved in an Ag ~(+) -mediated interaction with an A _(10) in a neighboring asymmetric unit, and did not interact with the Ag _(16) NC. Therefore, we synthesized AgNCs embedded in the corresponding 9-base sequence in order to investigate the crystal structure of these new DNA-A _(10) :Ag _(16) NCs and analyze the photophysical properties of the solution and crystalline state. The X-ray crystallography and spectroscopic measurements revealed that the 3′-end adenosine has little importance with respect to the photophysics and structure of the Ag _(16) NCs. Additionally, the new crystallographic data was recorded with higher spatial resolution leading to a more detailed insight in the interactions between the nucleotides and Ag atoms.
机译:呈上末端腺苷(A _(10))对先前描述的DNA稳定的Ag _(16)纳米簇(DNA:Ag _(16)Nc)的光谱和结构性质的作用。在原始DNA:Ag _(16)NCS(5'-cacctagccga-3')中,A _(10)核酸酶参与与邻近的A _(10)中的Ag〜(+)介导的相互作用不对称单元,并没有与AG _(16)NC相互作用。因此,我们嵌入在相应的9碱基序列中的合成AGNC,以研究这些新DNA-A _(10):Ag _(16)NC的晶体结构,并分析溶液和结晶状态的光物理性质。 X射线晶体学和光谱测量表明,3'-末端腺苷对Ag _(16)NCS的光学和结构几乎没有重要。另外,新的晶体数据以较高的空间分辨率记录,导致核苷酸和Ag原子之间的相互作用更详细的洞察。

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