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Amino-modified kraft lignin microspheres as a support for enzyme immobilization

机译：氨基改性的牛皮油木质素微球作为酶固定化的支持

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In this research, it has been demonstrated that amino-modified microspheres (A-LMS) based on bio-waste derived material, such as kraft lignin, have good prospects in usage as a support for enzyme immobilization, since active biocatalyst systems were prepared by immobilizing β-galactosidase from A. oryzae and laccase from M. thermophila expressed in A. oryzae (Novozym? 51003) onto A-LMS. Two types of A-LMS were investigated, with different emulsifier concentrations (5 wt% and 10 wt%), and microspheres produced using 5 wt% of emulsifier (A-LMS_5) showed adequate pore shape, size and distribution for enzyme attachment. The type of interactions formed between enzymes (β-galactosidase and laccase) and A-LMS_5 microspheres demonstrated that β-galactosidase is predominantly attached via electrostatic interactions while attachment of laccase is equally governed by electrostatic and hydrophobic interactions. Furthermore, the A-LMS_5-β-galactosidase exhibited specificity towards recognized prebiotics (galacto-oligosaccharides (GOS)) synthesis with 1.5-times higher GOS production than glucose production, while for environmental pollutant lindane degradation, the immobilized laccase preparation exhibited high activity with a minimum remaining lindane concentration of 22.4% after 6 days. Thus, this novel enzyme immobilization support A-LMS_5 has potential for use in green biotechnologies.
机译：在该研究中，已经证明基于生物废物衍生材料的氨基改性微球（A-LMS），例如牛皮纸，作为对酶固定化的支持，具有良好的应用前景，因为活性生物催化剂制备从A. Oryzae（Novozym？51003）中的M. Hotherophira从A.Mathercira从A.Craincophia（Novozym？51003）中的植物和漆酶固定β-半乳糖苷酶。研究了两种类型的A-LMS，用不同的乳化剂浓度（5wt％和10wt％），并且使用5wt％的乳化剂（a-lms_5）产生的微球显示出足够的孔形状，尺寸和酶附着的分布。酶（β-半乳糖苷酶和漆酶）和A-LMS_5微球之间形成的相互作用的类型证明了β-半乳糖苷酶主要通过静电相互作用连接，同时漆酶的附着同等地由静电和疏水相互作用。此外，A-LMS_5-β-半乳糖苷酶对公认的益生元（吡酰基 - 寡糖（GOS））合成具有比葡萄糖产生的1.5倍较高的GOS产生，而用于环境污染物林丹降解，则固定化的漆酶制剂表现出高活性6天后，最低含林丹浓度为22.4％。因此，这种新型酶固定化支持A-LMS_5具有在绿色生物技术中使用的潜力。

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