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Construction of gold-siRNANPR1 nanoparticles for effective and quick silencing of NPR1 in Arabidopsis thaliana

机译：拟合拟南芥NPR1的Gold-sirnanpr1纳米粒子的构建拟南芥

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In recent years, gold nanoparticles (AuNPs) have been widely used as gene silencing agents and therapeutics for treatment of cancers due to their high transfection efficiency and lack of cytotoxicity, but their roles in gene silencing in plants have not yet been reported. Here, we report synthesis of AuNPs-branched polyethylenimine and its integration with the small interfering RNAs (siRNA) of NPR1 to form a AuNPs-siRNA _( NPR1 ) compound. Our results showed that AuNPs-siRNA _( NPR1 ) was capable of infiltrating into Arabidopsis cells. AuNPs-siRNA _( NPR1 ) silenced 80% of the NPR1 gene in Arabidopsis . Bacteriostatic and ion leakage experiments suggest that the NPR1 gene in Arabidopsis leaves was silenced by AuNPs-siRNA _( NPR1 ) . In Columbia-0 plants, compared with the control group treated with buffer solution, the AuNPs-siRNA _( NPR1 ) treatment significantly increased the number of colonies and cell death, and the leaves turned yellow, similar to the phenotype of the npr1 leaves. These results indicated this AuNPs-siRNA _( NPR1 ) silencing the NPR1 gene method is simple, effective and quick (3 days), and a powerful tool to study gene functions in plants.
机译：近年来，由于其高转染效率和缺乏细胞毒性，金纳米颗粒（AUNP）已被广泛用作基因沉默剂和治疗癌症，但尚未报道它们在植物中的基因沉默中的作用。在此，我们报告了AuNPS支化的聚乙烯亚胺的合成及其与NPR1的小干扰RNA（siRNA）的整合以形成AUNPS-siRNA _（NPR1）化合物。我们的结果表明，AUNPS-siRNA _（NPR1）能够渗透到拟南芥细胞中。 AUNPS-siRNA _（NPR1）拟定拟南芥中的80％NPR1基因。抑菌和离子泄漏实验表明拟南芥叶中的NPR1基因由AUNPS-siRNA _（NPR1）静音。在哥伦比亚-0植物中，与用缓冲溶液处理的对照组相比，AUNPS-siRNA _（NPR1）处理显着增加了菌落和细胞死亡的数量，叶片变为黄色，类似于NPR1叶子的表型。这些结果表明，这种AuNPS-siRNA _（NPR1）沉默NPR1基因方法简单，有效，快速（3天），以及研究植物中基因功能的强大工具。

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