• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>RSC Advances >Isolation ssDNA aptamers specific for both live and viable but nonculturable state Vibrio vulnificus using whole bacteria-SEILEX technology
【24h】

Isolation ssDNA aptamers specific for both live and viable but nonculturable state Vibrio vulnificus using whole bacteria-SEILEX technology

机译：使用全细菌 - Seilex技术的活性和可行但不可培养的状态VibrioVibriafifus特异的分离SSDNA适体特异性

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Vibrio vulnificus is a ubiquitous marine bacterium that may cause rapid and deadly infection, threatening lives of people living around natural bodies of water, especially in coastal regions. However, traditional culture-based methods are time-consuming and unable to detect Viable But Non-Culturable (VBNC) V. vulnificus cells. In this work, we isolated a batch of detection aptamers specifically binding to V. vulnificus in all culture status. With traditional whole bacteria-SELEX (Systematic Evolution of Ligands by EXponential enrichment), flow cytometer analysis and imaging, we identify 18 candidates and validated two of them (V8 and V13) as applicable aptamers. Their truncated sequences also showed comparable performance. The dissociation constant (KD) value of V8 is shown to be as low as 11.22 ± 1.32 nM. Optimal aptamers V8 and V13 are also validated to be effective to detect different Vibrio vulnificus strains under different binding environments using flow cytometry. As for detection parameters, the LOD of the V8 from cytometry is 29.96 CFU mL ~(?1) , and the linear range is 10 ~(2) –5 × 10 ~(5) CFU mL ~(?1) . This is the first case demonstrating that aptamers can detect the existence of VBNC bacteria as well as live bacteria.
机译：vibrio wulnificus是一种普遍存在的海洋细菌，可能会导致快速和致命的感染，威胁生活在沿海地区的自然身体周围的自然体内的人们。然而，传统的培养方法是耗时的，无法检测到可行但非培养的（VBNC）V.Vulnificus细胞。在这项工作中，我们孤立一批特异性结合V.Vulnificus的批次检测适体。随着传统的整个细菌 - SELEX（通过指数富集的配体的系统演变），流动细胞仪分析和成像，我们鉴定了18名候选物并验证了其中两种（V8和V13），如适用的适体。它们截断的序列也显示出可比的性能。 V8的解离常数（Kd）值显示为低至11.22±1.32nm。还验证了最佳的适体V8和V13，以利用流式细胞术在不同的结合环境下检测不同的vibrio whraunfificus菌株。对于检测参数，来自细胞测定法的V8的床位是29.96 CFU mL〜（α1），线性范围为10〜（2）-5×10〜（5）CFU mL〜（α1）。这是第一种证明适体可以检测到VBNC细菌的存在以及活细菌的病例。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号