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首页> 外文期刊>RSC Advances >Three-dimensional cell printing of gingival fibroblast/acellular dermal matrix/gelatin–sodium alginate scaffolds and their biocompatibility evaluation in vitro
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Three-dimensional cell printing of gingival fibroblast/acellular dermal matrix/gelatin–sodium alginate scaffolds and their biocompatibility evaluation in vitro

机译：牙龈成纤维细胞/无细胞皮肤基质/明胶 - 海藻酸钠支架及其体外生物相容性评价的三维细胞印刷及其生物相容性评价

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Tissue engineering has emerged as a promising approach for soft tissue regeneration. Three-dimensional (3D) cell printing showed great potential for producing cell-encapsulated scaffolds to repair tissue defects. The advantage of 3D cell printing technology is precise cell loading in scaffolds to achieve tissue regeneration instead of only relying on the cells from surrounding tissue or blood. A new acellular dermal matrix/gelatin–sodium alginate (ADM/A/G) scaffold with living gingival fibroblasts was constructed by 3D cell printing technology for potential oral soft tissue regeneration in this study, and the biological characteristics of the 3D cell printing scaffolds were evaluated. The residue of nucleic acid and growth factors in ADM were detected. Three biomaterials were mixed at an appropriate radio with human gingival fibroblasts (hGFs) to prepare bioinks. Two kinds of layer scaffolds were fabricated by 3D cell printing technology. The mechanical strength and degradability of the scaffolds were determined by measuring their compressive modulus and mass loss. CCK-8 assay and calcein-AM/PI staining were conducted to detect the cell proliferation and viability in 3D cell printing scaffolds. The morphology of the hGFs in the scaffolds were observed using SEM and FITC-phalloidin staining. The expression of COL1A1, PECAM1, and VEGF-A of hGFs in the scaffolds were quantified by qRT-PCR. The gelatin–sodium alginate (A/G) scaffolds were used as control group in all experiments. Compared with the control group, 3D cell printing ADM/A/G scaffolds showed better mechanical strength and longer degradation time. The ADM/A/G scaffolds obviously had a better promotion effect on cell proliferation and viability. Most of the hGFs observed had a fully extended spindle morphology in the ADM/A/G scaffolds but oval morphology in the control group. The expression of COL1A1 was significantly higher than in the control group with time, and the expression of PECAM1 and VEGF-A was slightly higher in ADM/A/G scaffolds on day 14. 3D cell printing gingival fibroblast-ADM/A/G scaffolds showed excellent biological properties, which could be potentially useful in oral soft tissue regeneration.
机译：组织工程作为软组织再生的有希望的方法。三维（3D）细胞印刷显示出生产细胞包封支架以修复组织缺陷的巨大潜力。 3D细胞印刷技术的优点是支架中的精确细胞载荷，以实现组织再生，而不是仅依赖于周围组织或血液的细胞。通过用于潜在口腔软组织再生的3D细胞印刷技术，构建了一种新的无细胞皮肤基质/明胶 - 海藻酸钠（ADM / A / G）支架，具有用于潜在口腔软组织再生的3D细胞印刷技术，以及3D细胞印刷支架的生物学特性评估。检测核酸和生长因子的残留物。将三种生物材料与人牙龈成纤维细胞（HGF）的合适的无线电混合以制备生物纤维蛋白。通过3D细胞印刷技术制造两种层支架。通过测量它们的压缩模​​量和质量损失来确定支架的机械强度和可降解性。进行CCK-8测定和Calcein-AM / Pi染色以检测3D细胞印刷支架中的细胞增殖和活力。使用SEM和FITC-Phalloidin染色观察支架中HGFS的形态。通过QRT-PCR定量COL1A1，PECAM1和VEGF-A的HGFS的表达。所有实验中使用明胶 - 藻酸钠（A / G）支架作为对照组。与对照组相比，3D细胞印刷ADM / A / G支架显示出更好的机械强度和更长的降解时间。 ADM / A / G支架显然对细胞增殖和活力具有更好的促进作用。观察到的大多数HGF在ADM / A / G支架中具有完全延伸的主轴形态，但对照组中的卵形形态。 Col1a1的表达明显高于对照组随时间的时间，并且在第14天的AD / A / G支架上的PECAM1和VEGF-A的表达略高。3D细胞印刷牙龈成纤维细胞-AM / A / G支架显示出优异的生物学性质，这可能在口腔软组织再生中可能有用。

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