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首页> 外文期刊>RSC Advances >Core size does not affect blinking behavior of dye-doped Ag@SiO2 core–shell nanoparticles for super-resolution microscopy
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Core size does not affect blinking behavior of dye-doped Ag@SiO2 core–shell nanoparticles for super-resolution microscopy

机译：核心尺寸不会影响染料掺杂Ag〜SiO2核 - 壳纳米粒子的闪烁行为，用于超分辨率显微镜

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Dye-doped nanoparticles have been investigated as bright, luminescent labels for super-resolution microscopy via localization methods. One key factor in super-resolution is the size of the luminescent label, which in some cases results in a frame shift between the label target and the label itself. Ag@SiO _(2) core–shell nanoparticles, doped with organic fluorophores, have shown promise as super-resolution labels. One key aspect of these nanoparticles is that they blink under certain conditions, allowing super-resolution localization with a single excitation source in aqueous solution. In this work, we investigated the effects of both the Ag core and the silica (SiO _(2) ) shell on the self-blinking properties of these nanoparticles. Both core size and shell thickness were manipulated by altering the reaction time to determine core and shell effects on photoblinking. Size and shell thickness were investigated individually under both dry and hydrated conditions and were then doped with a 1 mM solution of Rhodamine 110 for analysis. We observed that the cores themselves are weakly luminescent and are responsible for the blinking observed in the fully-synthesized metal-enhanced fluorescence nanoparticles. There was no statistically significant difference in photoblinking behavior—both intensity and duty cycle—with decreasing core size. This observation was used to synthesize smaller nanoparticles ranging from approximately 93 nm to 110 nm as measured using dynamic light scattering. The blinking particles were localized via super-resolution microscopy and show single particle self-blinking behavior. As the core size did not impact blinking performance or intensity, the nanoparticles can instead be tuned for optimal size without sacrificing luminescence properties.
机译：已经研究了染料掺杂的纳米颗粒作为通过定位方法的超分辨率显微镜的明亮的发光标记。超分辨率的一个关键因素是发光标签的大小，在某些情况下，在某些情况下导致标签目标和标签本身之间的帧移位。 Ag @ SiO_（2）掺杂有机荧光团的核 - 壳纳米粒子，并作为超级分辨率标签所示。这些纳米颗粒的一个关键方面是它们在某些条件下闪烁，使得具有在水溶液中的单一激发源的超分辨率定位。在这项工作中，我们研究了Ag核和二氧化硅（SiO _（2））壳对这些纳米颗粒的自闪透性质的影响。通过改变反应时间来确定核心尺寸和壳厚度，以确定对光照的核心和壳体效应。在干燥和水合条件下单独研究大小和壳厚度，然后用1mM的罗丹明110溶液进行分析。我们观察到芯本身是弱发光的，并且负责在完全合成的金属增强荧光纳米粒子中观察到的闪烁。光照行为 - 强度和占空比没有统计学上的显着差异 - 核心尺寸减小。使用使用动态光散射测量的测量，该观察用于合成范围为约93nm至110nm的较小纳米颗粒。闪烁的颗粒通过超分辨率显微镜局部定位，并显示单颗粒自闪烁行为。由于核心尺寸没有影响闪烁的性能或强度，因此纳米颗粒可以在不牺牲发光特性的情况下进行纳米颗粒以获得最佳尺寸。

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