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首页> 外文期刊>RSC Advances >Comprehensive metabolomics analysis based on UPLC-Q/TOF-MSE and the anti-COPD effect of different parts of Celastrus orbiculatus Thunb.
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Comprehensive metabolomics analysis based on UPLC-Q/TOF-MSE and the anti-COPD effect of different parts of Celastrus orbiculatus Thunb.

机译：基于UPLC-Q / TOF-MSE的综合性代谢组合分析及Celastrus Orbiculatus Thunb不同部分的反COPD效果。

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摘要

The root, stem and leaf of Celastrus orbiculatus Thunb. (COT) have all been used as Chinese folk medicine. Aiming at revealing the secondary metabolites and screening the anti-COPD effect of COT, the comprehensive phytochemical and bioassay studies were performed. Based on the ultra-high performance liquid chromatography combined with quadrupole time-of-flight mass spectrometry (UPLC-Q/TOF-MS ~(E) ), the screening analysis of components in COT was conducted with the UNIFI platform, the metabolomics of the three parts were analyzed with multivariate statistical analysis. Cigarette smoke extract (CSE)-stimulated inflammatory model in A549 cells was used to investigate the biological effect of the three parts. A total of 120 compounds were identified or tentatively characterized from COT. Metabolomics analysis showed that the three parts of COT were differentiated, and there were 13, 8 and 5 potential chemical markers discovered from root, stem and leaf, respectively. Five robust chemical markers with high responses could be used for further quality control in different parts of COT. The root, stem and leaf of COT could evidently reduce the levels of pro-inflammatory factors in a dose-dependent way within a certain concentration range. The stem part had a stronger anti-COPD effect than root and leaf parts. This study clarified the structural diversity of secondary metabolites and the various patterns in different parts of COT, and provided a theoretical basis for further utilization and development of COT.
机译：Celastrus orbiculatus thunb的根，茎和叶子。 （婴儿床）都被用作中国民间医学。旨在揭示次级代谢产物并筛选婴儿床的抗COPD效应，进行综合植物化学和生物测定研究。基于超高效液相色谱相结合四极杆飞行时间质谱（UPLC-Q / TOF-MS〜（E）），用UNIFI平台进行COT中组分的筛选分析，代谢组用多元统计分析分析三个部分。 A549细胞中的香烟烟雾提取物（CSE）-Acticuld炎症模型用于研究三个部分的生物学效果。鉴定了120种化合物或暂时用婴儿床表征。代谢组科分析表明，分别是分化的三个部分，分别从根，茎和叶中发现了13,8和5个潜在的化学标志物。具有高响应的五种具有高响应的强大化学标记可用于在COT的不同部分中进行进一步的质量控制。 COT的根，茎和叶可以明显降低在一定浓度范围内以剂量依赖性方式降低促炎因子的水平。茎部具有比根和叶件更强的抗COPD效果。本研究阐明了次级代谢物的结构多样性和婴儿床不同部位的各种模式，并为进一步利用和发育提供了理论依据。

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