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首页> 外文期刊>RSC Advances >Amplified piezoelectrically actuated on-chip flow switching for a rapid and stable microfluidic fluorescence activated cell sorter
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Amplified piezoelectrically actuated on-chip flow switching for a rapid and stable microfluidic fluorescence activated cell sorter

机译：用于快速和稳定的微流体荧光活性细胞分选仪的缓释压电驱动的片上流动切换

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With the potential to avoid cross-contamination, eliminate bio-aerosols, and minimize device footprints, microfluidic fluorescence-activated cell sorting (μ-FACS) devices could become the platform for the next generation cell sorter. Here, we report an on-chip flow switching based μ-FACS mechanism with piezoelectric actuation as a fast and robust sorting solution. A microfluidic chip with bifurcate configuration and displacement amplified piezoelectric microvalves has been developed to build the μ-FACS system. Rare fluorescent microparticles of different sizes have been significantly enriched from a purity of ～0.5% to more than 90%. An enrichment of 150-fold from ～0.6% to ～91% has also been confirmed for fluorescently labeled MCF-7 breast cancer cells from Jurkat cells, while viability after sorting was maintained. Taking advantage of its simple structure, low cost, fast response, and reliable flow regulation, the proposed μ-FACS system delivers a new option that can meet the requirements of sorting performance, target selectivity, device lifetime, and cost-effectiveness of implementation.
机译：借助于避免交叉污染，消除生物气溶胶，并最小化器件占地面积，微流体荧光激活的细胞分选（μ-FACS）器件可以成为下一代电池分拣机的平台。在这里，我们报告了基于电压切换的基于电压切换机构，具有压电致动作为快速且坚固的分选解决方案。已经开发出具有分叉配置和位移放大的压电微纤维的微流体芯片以构建μ-FACS系统。罕见的不同尺寸的稀有荧光微粒已显着富含〜0.5％至90％以上的纯度。富含〜0.6％至约91％的富集150倍至〜91％的富集，对于来自Jurkat细胞的荧光标记的MCF-7乳腺癌细胞，而在分选后的活力。利用其简单的结构，低成本，快速响应和可靠的流量调节，提出的μ-FACS系统提供了一种新选项，可以满足性能，目标选择性，设备寿命和实现成本效益的要求。

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