Detection of signal molecules in living plants is of great relevance for precision farming. In this work, to establish a more effective method for monitoring salicylic acid (SA) in the leaves of living plants, a ratiometric electrochemical sensor was fabricated based on a Cu metal–organic framework (Cu-MOF) and carbon black (CB) composite. The Cu-MOF and CB composite was used to catalyze SA oxidation. Ratiometric oxidation current peak intensities I _(SA) / I _(Cu-MOFs) were used as the response signal for SA. I _(SA) / I _(Cu-MOFs) linearly enhanced with the increase of SA concentration, together with low limits of detection (12.50 μM). Moreover, our sensor is fabricated on a screen-printed electrode (SPE), which is especially suitable for applying to the flat leaves of plants. Using this sensor, the SA level in the leaves of cucumber seedlings was monitored in vivo under salt stress. The proposed sensor is accurate, reliable and practical. This is the first report for developing a ratiometric electrochemical sensor for detecting SA in living plants. Our work can also provide a strategy for in vivo studies on the leaves of plants.
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机译：生物植物中信号分子的检测与精密养殖具有很大的相关性。在这项工作中，为了建立一种更有效的方法，用于在生物植物的叶片中监测水杨酸（SA），基于Cu金属 - 有机骨架（Cu-MOF）和炭黑（CB）复合材料制造比例电化学传感器。使用Cu-MOF和CB复合材料催化SA氧化。比率氧化电流峰强度I _（SA）/ I _（CU-MOF）用作SA的响应信号。 I _（SA）/ I _（CU-MOF）随着SA浓度的增加而线性增强，以及低检测限（12.50μm）。此外，我们的传感器在丝网印刷电极（SPE）上制造，其特别适用于施加到植物的平坦叶子。使用该传感器，在盐胁迫下体内监测黄瓜幼苗的叶片中的SA水平。所提出的传感器准确，可靠且实用。这是用于开发用于检测活植物中SA的比率电化学传感器的第一个报告。我们的工作还可以为植物叶子的体内研究提供一种策略。
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