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Lanthanide chelate-encapsulated polystyrene nanoparticles for rapid and quantitative immunochromatographic assay of procalcitonin

机译:用于快速和定量免疫层状测定的镧系元素螯合物包封的聚苯乙烯纳米粒子(ProCalcitonin)

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Procalcitonin (PCT) is a potentially specific early marker of bloodstream infection and sepsis. In this work, we report, for the first time, the development of a fluorescence-based immunochromatographic strip test (IST) which employs Eu(DBM) _(3) phen-containing nanoparticles as reporters for the detection of PCT in human serum. Experimental results demonstrate that the developed IST has an excellent ability for rapid (15 min), sensitive (0.05 ng mL ~(?1) ) and quantitative analysis of PCT, and the assay results are comparable to which of the conventional enzyme-linked immunosorbent assay (ELISA). These results suggest that the developed assay could be utilized for point-of-care detection of PCT.
机译:ProCalcitonin(PCT)是血流感染和败血症的潜在特异性早期标记。在这项工作中,我们首次报告的荧光基免疫层析条试验(IST),其使用EU(DBM)_(3)烷基纳米粒子作为用于检测人血清中PCT的记者。实验结果表明,发达的IST具有优异的快速(15分钟),敏感(0.05 ng mL〜(α1))和PCT的定量分析,并且测定结果与常规酶联免疫吸附的定量分析测定(ELISA)。这些结果表明,开发的测定可用于PCT的护理点检测。

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