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首页> 外文期刊>RSC Advances >Electrochemical sensor for individual and simultaneous determination of guanine and adenine in biological fluids and in DNA based on a nano-In–ceria modified glassy carbon paste electrode
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Electrochemical sensor for individual and simultaneous determination of guanine and adenine in biological fluids and in DNA based on a nano-In–ceria modified glassy carbon paste electrode

机译：基于纳米内改性玻碳浆料电极的个体和同时测定鸟嘌呤和DNA中鸟嘌呤和腺嘌呤的电化学传感器

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摘要

A novel, simple and sensitive electrochemical method for individual and simultaneous determination and direct electro-oxidation behaviors of guanine (G) and adenine (A) was developed using an In doped ceria nanoparticle modified glassy carbon paste electrode (In–CeO _(2) /GCPE). Scanning electron microscopy (SEM) and electrochemical techniques were employed for characterization of the fabricated modified electrode. The electrochemical sensor exhibits a potent and persistent electro-oxidation behavior followed by well-separated oxidation peaks toward G and A. Electrochemical performances related to the electro-oxidation of G and A at the nano-In–CeO _(2) NPs/GCPE were investigated, showing that their peak currents were greatly enhanced due to the catalytic effect of In doped ceria. The prepared In–CeO _(2) NPs/GCPE showed high selectivity and sensitivity for G and A oxidation over uric acid (UA). A linear range of 0.07–34 μM with a detection limit of 1.19 × 10 ~(?8) M for G and 1.96–88.2 μM with a detection limit of 2.86 × 10 ~(?8) M for A were achieved. The proposed sensor was used for individual and simultaneous determination of G and A in biological fluids and in ssDNA samples. The value of (G + C)/(A + T) in DNA was calculated to be 0.75.
机译：使用掺杂的二氧化铈纳米粒子改性玻璃碳浆电极（CEO _（2）中的一种新型和同时测定和腺嘌呤（A）的个体和同时测定和直接电氧化行为和直接电氧化行为的新颖，简单敏感的电化学方法（G）和腺嘌呤（A） / GCPE）。扫描电子显微镜（SEM）和电化学技术用于表征制造的修饰电极。电化学传感器表现出有效的和持续的电氧化行为，然后朝向G和A的良好分离的氧化峰和A.与纳米CeO _（2）NPS / GCPE的电氧化有关的电化学性能研究了，表明它们的峰值电流由于掺杂的二氧化铈的催化作用而大大提高。制备的in-CeO _（2）NPS / GCPE显示出高选择性和敏感性，对尿酸（UA）氧化。对于G和1.96-88.2μm的检测限为1.19×10〜（Δ8）m的线性范围为1.19×10〜（？8）m，检测限为2.86×10〜（？8）m。所提出的传感器用于在生物流体中的单独和同时测定G和A中的含量和SSDNA样品。将DNA中的（G + C）/（A + T）的值计算为0.75。

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