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首页> 外文期刊>RSC Advances >A new route for SERS analysis of intact erythrocytes using polydisperse silver nanoplatelets on biocompatible scaffolds
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A new route for SERS analysis of intact erythrocytes using polydisperse silver nanoplatelets on biocompatible scaffolds

机译：在生物相容性支架上使用多分散银纳米粒子的完整红细胞SERS分析的新途径

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摘要

Superior spectral sensitivity and functional abilities of anisotropic, instead of the usually used spherical, noble metal nanoparticles, allow development of new surface-enhanced Raman spectroscopy (SERS) approaches to analyse biological objects. We found and resolved for the first time the particular risks of survival of silver nanoparticles in different salines to succeed in recording the SERS spectra of intact erythrocytes as an important family of living cells. The ensemble of nanoplatelets with varied shapes and sizes grants multispectral absorption of laser irradiation since a fraction of the nanoparticles with a given position of a plasmonic band always exists in such a mixture, thereby providing an effective SERS amplification. At the same time, fast recrystallization of anisotropic silver nanoplatelets occurs in a standard chloride-based saline, being important to keep the erythrocytes alive but neglecting the benefits of the silver platelets as the most versatile and prospective components of SERS sensors. Substitution of chlorides with nitrates keeps both the intact cells and anisotropic nanoparticles safe on biocompatible cellulose SERS scaffolds containing the mixture of silver nanoplatelets thus promoting the development of new SERS devices for biomedical diagnostics.
机译：各向异性的卓越的光谱敏感性和功能能力，而不是通常使用的球形贵金属纳米颗粒，允许开发新的表面增强拉曼光谱（SERS）接近分析生物物体的方法。我们首次发现并解决了不同盐水中银纳米粒子的存活的特殊风险，以成功地将完整红细胞的SERS光谱记录为一个重要的活细胞家族。具有变化的形状和尺寸的纳米孔的集合授予激光照射的多光谱吸收，因为具有等离子体带的给定位置的纳米颗粒的一部分总是存在于这种混合物中，从而提供有效的SERS扩增。同时，各向异性银纳米片的快速重结晶在标准的氯化物基盐水中发生，重要的是保持红细胞活着，但忽略了银血小板的益处作为SERS传感器的最通用和预期组分。用硝酸盐的氯化物取代完整的细胞和各向异性纳米颗粒在生物相容的纤维素SERS支架上保证，含有银纳米纳米纳米键的混合物，从而促进新的SERS设备进行生物医学诊断。

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